研究室オリジナルHP

生化学研究室

研究テーマ

糖鎖の機能解析と糖鎖異常による病気の発症機構の解析

最近、糖鎖の合成異常や欠損による様々な疾患が発見され、糖鎖が必須の役割を果たすことが明らかとなってきました。生化学研究室では、生化学的ならびに分子生物学的手法を用いて、糖鎖の機能解明に取り組んでいます。

大学院生募集


第三の生命鎖として注目されている糖鎖について、私たちと一緒に研究してみませんか?
下記に示すように経済的な支援制度も充実しています。

神戸薬科大学大学院奨学生規程による奨学生制度
授業料相当額を給付

日本学生支援機構大学院奨学生(貸与)
神戸薬科大学リサーチ・アシスタント規程によるリサーチ・アシスタント制度

詳しくは下記の URL を参照ください
http://kpubiochem.firebird.jp/研究内容/大学院生募集-2/

問い合わせ先


biochem@kobepharma-u.ac.jp

スタッフ

北川 裕之

北川 裕之 教授

担当科目:分子生物学I 生化学III 生物学系I実習 生命科学特論(大学院)
学位:博士(薬学)

灘中 里美

灘中 里美 准教授

担当科目:分子生物学II 生物学系I実習 生命科学特論(大学院)
学位:博士(薬学)

三上 雅久

三上 雅久 講師

担当科目:生化学II 生化学III 生物学系I実習 生命科学特論(大学院)
学位:博士(薬学)

内藤 裕子

内藤 裕子 特任助教

研究成果

【特許】
Hiroshi KITAGAWA, Tadahisa MIKAMI (2014. 04. 22)
PCT/JP2012/073794 「SKELETAL MUSCLE REGENERATION PROMOTER」

北川裕之,小池敏靖(2013.02.20)
特願 2013-031563「骨密度増加剤及び骨密度増加剤のスクリーニング方法」

北川裕之,灘中里美,田村純一(2013.03.29)
特願 2013-075157「ヘパラナーゼ阻害剤及びヘパラナーゼ阻害剤のスクリーニング方法」

【論文】

 2018


Matsushita, K., Nakata, T., Takeda-Okuda, N., Nadanaka, S., Kitagawa, H., and Tamura, J. (2018) Synthesis of chondroitin sulfate CC and DD tetrasaccharides and interactions with 2H6 and LY111. Bioorg. Med. Chem. in press. doi.org/10.1016/j.bmc.2018.01.011

Miyata, S., Nadanaka, S., Igarashi, M., and Kitagawa, H. (2018) Structural variation of chondroitin sulfate chains contributes to molecular heterogeneity of perineuronal nets. Front. Integr. Neurosci. 12, 3. doi: 10.3389/fnint.2018.00003

Nadanaka, S. and Kitagawa, H. (2018) Exostosin-like 2 regulates FGF2 signaling by controlling the endocytosis of FGF2. Biocim. Biophys. Acta 1862, 791-799.  doi:10.1016/j.bbagen.2018.01.002

Nadanaka, S., Kinouchi, H., and Kitagawa, H. (2018) Chondroitin sulfates-mediated N-cadherin/β-catenin signaling associated with basal-like breast cancer cell invasion.J. Biol. Chem., 293, 444-465 doi:10.1074/jbc.M117.814509

 2017


Yoshioka, N., Miyata, S., Tamada, A., Watanabe, Y., Kawasaki, A., Kitagawa, H., Takao, K., Miyokawa, T., Takeuchi, K., and Igarashi, M. (2017) Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis. Mol. Brain, 10, 47. DOI:10.1186/s13041-017-0328-5

Hou, X., Yoshioka, N., Tsukano, H., Sakai, A., Miyata, S., Watanabe, Y., Yanagawa, Y., Sakimura, K., Takeuchi, K., Kitagawa, H., Hensch, T., Shibuki, K., Igarashi, M., and Sugiyama, S. (2017) Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex.Sci. Rep., 7, 12646.doi:10.1038/s41598-017-04007-x

Yang, S., Hilton, S., Alves, J. N., Saksida, L. M., Bussey, T. M., Matthews, R. T., Kitagawa, H., Spillantini, M. G., Kwok, J. C. F., and Fawcett, J. W. (2017) Antibody recognising 4-sulphated chondroitin sulphate proteoglycans restores memory in tauopathy-induced neurodegeneration. Neurobiol. Aging, 59, 197-209. DOI: 10.1016/j.neurobiolaging.2017.08.002

Miyata, S., and Kitagawa, H. (2017) Formation and remodeling of the brain extracellular matrix in neural plasticity: roles of chondroitin sulfate and hyaluronan. Biochim. Biophys. Acta, 1861 (10), 2419-2432. doi:10.1016/j.bbagen.2017.06.010

Takeda, N., Ueda, M., Mori, N., Miyoshi, T., Shimoda, M., Uno, Y., Kitagawa, H., Emoto, N., Mukai, T., and Miyata, O. (2017) Fluorescence quenching induced by sequential addition-aromatization of a bodily-containing dienylimine with thiols. Heterocycles, 94, 750-762. doi: 10.3987/COM-16-13643

Shida, M., Mikami, T., Tamura, J., and Kitagawa, H. (2017) A characteristic chondroitin sulfate trisaccharide unit with a sulfated fucose branch exhibits neurite outgrowth-promoting activity: Novel biological roles of fucosylated chondroitin sulfates isolated from the sea cucumber Apostichopus japonicas. Biochem. Biophys. Res. Commun., 487, 678-683doi:10.1016/j.bbrc.2017.04.114

Mikami, T., and Kitagawa, H. (2017) Sulfated glycosaminoglycans: their distinct roles in stem cell biology. Glycoconjugate J., 34, 725-735. doi:10.1007/s10719-016-9732-9

灘中 里美、北川 裕之(2017) 「がんの発生と進行に関わるヘパラン硫酸」生化学 第89巻 第5号 pp. 681-688 doi:10.14952/SEIKAGAKU.2017.890681

 2010-2016


2016

Nadanaka, S., Kinouchi, H., Kitagawa, H. (2016) Histone Deacetylase-mediated Regulation of Chondroitin 4-O-sulfotransferase-1 (Chst11) Gene Expression by Wnt/β-catenin Signaling. Biochem. Biophys. Res. Commun. 480, 234-240doi: 10.1016/j.bbrc.2016.10.035

Izumikawa, T., Dejima, K., Watamoto, Y., Nomura, K. H., Kanai, N., Rikitake, M., Tou, M., Murata, D., Yanagita, E., Kano, A., Mitani, S., Nomura, K., and Kitagawa, H. (2016) Chondroitin 4-O-sulfotransferase is indispensable for sulfation of chondroitin and plays an important role in maintaining normal life span and oxidative stress responses in nematodes. J. Biol. Chem., 291 (44), 23294-23304. doi:10.1074/jbc.M116.757328

Sorg, B. A., Berretta, S., Blacktop, J. M., Fawcett, J. W., Kitagawa, H. Kwok, J. C. F., and Miquel, M. (2016) Casting a wide net: role of perineuronal nets in neural plasticity. J. Neurosci., 36 (45), 11459-11468. DOI: http://dx.doi.org/10.1523/JNEUROSCI.2351-16.2016

Takase, H., Tanaka, M., Yamamoto, A., Watanabe, S., Takahashi, S., Nadanaka, S., Kitagawa, H., Yamada, T., and Mukai, T. (2016) Structural requirements of glycosaminoglycans for facilitating amyloid fibril formation of human serum amyloid A. Amyloid, 23 (2) 67-75. doi: 10.3109/13506129.2016.1168292.

Miyata, S., and Kitagawa, H. (2016) Chondroitin sulfate and neuronal disorders. Front. Biosci., 21 (7) 1330-1340. dx.doi.org/10.2741/4460

Saigoh, K., Yoshimura, S., Izumikawa, T., Miyata, S., Tabara, Y., Matsushita, T., Miki, T., Miyamoto, K., Hirano, M., Kitagawa, H., Kita, J., and Kusunoki, S. (2016) Chondroitin sulfate β-1,4-N-acetylgalactosaminyltransferase-1 (ChGn-1) polymorphism; association with progression of multiple sclerosis. Neurosci. Res., 108, 55-59. doi:10.1016/j.neures.2016.01.002.

Miyata, S., and Kitagawa, H. (2016) Chondroitin 6-sulfation regulates perineuronal net formation by controlling the stability of aggrecan. Neural Plast., 2016, 1305801, 13 pages. dx.doi.org/10.1155/2016/1305801

Kinouchi, H., Matsuyama, K., Kitagawa, H., and Kamimori, H. (2016) Surface Plasmon Resonance Assay of Inhibition by Pharmaceuticals for Thyroxine Hormone Binging to Transport Proteins. Anal. Biochem., 492, 43-48. doi: 10.1016/j.ab.2015.09.004.

2015

Yabuno, K., Morise, J., Kizuka, Y., Hashii, N., Kawasaki N., Takahashi, S., Miyata, S., Izumikawa, T., Kitagawa, H., Takematsu, H., and Oka, S. (2015) A sulfated glycosaminoglycan linkage region is a novel type of Human Natural Killer-1 (HNK-1) epitope expressed on aggrecan in perineuronal nets.
PLoS ONE, 10 (12), e0144560. doi: 10.1371/journal.pone.0144560.

Yutsudo, N., and Kitagawa, H. (2015) Involvement of chondroitin 6-sulfation in temporal lobe epilepsy. Exp. Neurol., 274 Part B, 126-133. doi: 10.1016/j.expneurol.2015.07.009.

Capurro, M., Shi, W., Izumikawa, T., Kitagawa, H., and Filmus, J. (2015) Processing by Convertases Is Required for Glypican-3-induced Inhibition of Hedgehog Signaling. J. Biol. Chem., 290 (12) 7576-7585. doi: 10.1074/jbc.M114.612705.

Koike, T., Mikami, T., Shida, M., Habuchi, O., and Kitagawa, H. (2015) Chondroitin sulfate-E mediates estrogen-induced osteoanabolism. Sci. Rep., 5, 8994. doi:10.1038/srep08994

Izumikawa, T., and Kitagawa, H. (2015) Amino acid sequence surrounding the chondroitin sulfate attachment site of thrombomodulin regulates chondroitin polymerization. Biochem. Biophys. Res. Commun., 460 (2) 233-237. doi: 10.1016/j.bbrc.2015.03.016.

Tomatsu, S., Shimada, T., Patel, P., Mason, R. W., Mikami, T., Kitagawa, H., Montano, A. M., and Orii, T. (2015) Chondroitin and keratan sulfate. Sulfated Polysaccharides (Gama, M., Nader, H., and de Oliveira Rocha, H., eds) pp. 17-71, Nova Science Publishers.

Izumikawa, T., Sato, B., Mikami, T., Tamura, J., Igarashi, M., and Kitagawa, H. (2015) GlcUAβ1-3Galβ1-3Galβ1-4Xyl(2-O-phosphate) is the preferred substrate for chondroitin N-acetylgalactosaminyltransferase-1. J. Biol. Chem., 290 (9) 5438-5448. doi: 10.1074/jbc.M114.603266.

Mikami, T., and Kitagawa, H. (2015) Glycan structure and neural plasticity. Sugar chains (Taniguchi, N., Suzuki, T., and Ohtsubo, K., eds) pp. 107-126, Springer.

Taniguchi, M., Nadanaka, S., Tanakura, S., Sawaguchi, S., Midori, S., Kawai, Y., Yamaguchi, S., Shimada, Y., Nakamura, Y., Matsumura, Y., Fujita, N., Araki, N., Yamamoto, M., Oku, M., Wakabayashi, S., Kitagawa, H., and Yoshida, H. (2015) TFE3 is a bHLH-ZIP-type transcription factor that regulates the mammalian Golgi stress response. Cell Struct. Funct., 40(1) 13-30. (CSF Award) doi: 10.1247/csf.14015.

Miyata, S., and Kitagawa, H. (2015) Mechanisms for modulation of neural plasticity and axon regeneration by chondroitin sulfate. J. Biochem., 157 (1) 13-22. doi: 10.1093/jb/mvu067.

【図書】三上雅久、北川裕之 第5章 糖質 スタンダード薬学シリーズII 4 生物系薬学 I. 生命現象の基礎(東京化学同人)pp. 28-34 (2015)

【図書】灘中 里美, 北川 裕之「糖鎖の新機能開発•応用ハンドブック 創薬•医療から食品開発まで」(NTS 双文社印刷) pp.36-40(2015) 第1章 生体内の糖鎖 第3節 プロテオグリカン ①コンドロイチン硫酸

2014

Kitagawa, H. (2014) Using sugar-remodeling to study chondroitin sulfate function. Biol. Pharm. Bull., 37 (11) 1705-1712.

Kinouchi, H., Arimoto, H., Nishiguchi, K., Oka, M., Maki, H., Kitagawa, H., and Kamimori, H. (2014) Binding Properties of Antimicrobial Agents to Lipid Membranes Using Surface Plasmon Resonance. Biol. Pharm. Bull., 37 (8) 1383-1389.

Nadanaka, S., and Kitagawa, H. (2014) EXTL2 controls liver regeneration and aortic calcification through xylose kinase-dependent regulation of glycosaminoglycan biosynthesis. Matrix Biol., 35, 18-24. doi: 10.1016/j.matbio.2013.10.010.

Nadanaka, S., Purunomo, E., Takeda, N., Tamura, J., and Kitagawa, H. (2014) Heparan Sulfate Containing Unsubstituted Glucosamine Residues: Biosynthesis and Heparanase Inhibitory Activity. J. Biol. Chem., 289(22), 15231-15243. (Faculty of 1000) doi: 10.1074/jbc.M113.545343.

Mikami, T., and Kitagawa, H. (2014) Glycosaminoglycans: their modes of action for a possible new avenue for therapeutic intervention. Glycoscience: Biology and Medicine (Taniguchi, N., Endo, T., Hart, G.W., Seeberger, P.H., and Wong, C.H., eds) pp. 511-517, Springer.

Miyamoto, K., Tanaka, N., Moriguchi, K., Ueno, R., Kadomatsu, K., Kitagawa, H., and Kusunoki, S. (2014) Chondroitin 6-O-sulfate ameliorates experimental autoimmune encephalomyelitis. Glycobiology, 24 (5), 469-475. doi: 10.1093/glycob/cwu014.

Nadanaka, S., and Kitagawa, H. (2014) Beta-1,3-glucuronyltransferase 3 (glucuronyltransferase I) (B3GAT3). Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp. 849-861, Springer.

Nadanaka, S., and Kitagawa, H. (2014) Exostosin (multiple)-like 1-3 (EXTL1-3). Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp. 885-903, Springer.

Nadanaka, S., and Kitagawa, H. (2014) Exostosin 1 and 2 (EXT1, 2). Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp. 905-923, Springer.

Nadanaka, S., and Kitagawa, H. (2014) Chondroitin polymerizing factor, chondroitin polymerizing factor 2, chondroitin sulfate synthase 1, 3 (CHPF, CHPF2, CHSY1, CHSY3). Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp. 947-963, Springer.

Kinouchi, H., Arimoto, H., Nishiguchi, K., Oka, M., Maki, H., Kitagawa, H., and Kamimori, H. (2014) Binding properties of antimicrobial agents to dipeptide terminal of lipid II using surface Plasmon resonance. Anal. Biochem., 452, 67-75. doi: 10.1016/j.ab.2014.02.012.

Koike, T., Izumikawa, T., Sato, B., and Kitagawa, H. (2014) Identification of phosphatase that dephosphorylates xylose in the glycosaminoglycan-protein linkage region of proteoglycans. J. Biol. Chem., 289 (10), 6695-6708. (Faculty of 1000) doi: 10.1074/jbc.M113.520536.

Izumikawa, T., Sato, B., and Kitagawa, H. (2014) Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells. Sci. Rep., 4, 3701. doi: 10.1038/srep03701.

2013

Takeuchi, K., Yoshioka, N., Higa Onaga, S., Watanabe, Y., Miyata, S., Wada, Y., Kudo, C., Okada, M., Ohko, K., Oda, K., Sato, T., Yokoyama, M., Matsushita, N., Nakamura, M., Okano, H., Sakimura, K., Kawano, H., Kitagawa, H., and Igarashi, M. (2013) Chondroitin sulphate N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury.
Nature Commun. 4, 2740.

Miyoshi, T., Aoki, Y., Uno, Y., Araki, M., Kamatani, T., Fujii, D., Fujita, Y., Takeda, N., Ueda, M., Kitagawa, H., Emoto, N., Mukai, T., Tanaka, M., and Miyata, O. (2013) Michael Addition-Aromatization Reaction of Dienylimines Bearing a Leaving Group and its Application to the Preparation of Thiol Selective Labeling Reagents Capable of Forming Strong Carbon-Sulfur Bonds. J. Org. Chem., 78 (22), 11433-11443.

Purunomo, E., Emoto, N., Nugrahaningsih, D., Nakayama, K., Yagi, K., Heiden, S., Nadanaka, S., Kitagawa, H., and Hirata, K. (2013) Glycosaminoglycan overproduction in the aorta increases aortic calcification in murine chronic kidney disease. J. Am. Heart Assoc., 2 (5), e000405.

Izumikawa, T., Saigoh, K., Shimizu, J., Tsuji, S., Kusunoki, S., and Kitagawa, H. (2013) A chondroitin synthase-1 (ChSy-1) missense mutation in a patient with neuropathy impairs the elongation of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1. Biochim. Biophys. Acta, 1830 (10), 4806-4812.

Mikami, T., and Kitagawa, H. (2013) Biosynthesis and function of chondroitin sulfate. Biochim. Biophys. Acta, 1830 (10), 4719-4733.

Nadanaka, S., Kagiyama, S., and Kitagawa, H. (2013) Roles of EXTL2, a member of EXT family of tumor suppressors, in liver injury and regeneration processes. Biochem. J., 454 (1), 133-145.

Jaureguiberry, G., De la Dure-Molla, M., Parry, D., Quentric, M., Himmerkus, N., Koike, T., Poulter, J., Klootwijk, E., Robinette, S. L., Howie, A. J., Patel, V., Figueres, M. -L., Stanescu, H. C., Issler, N, Nicholson, J. K., Bockenhauer, D., Laing, C., Walsh, S. B., McCredie, D. A., Povey, S., Asselin, A, Picard, A., Coulomb, A., Medlar, A. J., Bailleul-Forestier, I., Verloes, A,. Le Caignec, C., Roussey, G., Guiol, J., Isidor, B., Logan, C., Shore, R., Johnson, C., Inglehearn, C., Al-Bahlani, S., Schmittbuhl, M., Clauss, F., Huckert, M., Laugel, V., Ginglinger, E., Pajarola, S., Sparta, G., Bartholdi, D., Rauch, A., Addor, M. -C., Yamaguti, P. M., Safatle, H. P., Acevedo, A. C., Martelli-Junior, H., dos Santos Netos, P. E., Coletta, R. D., Gruessel, S., Sandmann, C., Ruehmann, D., Langman, C. B., Scheinman, S. J., Ozdemir-Ozenen, D., Hart, T. C., Hart, P. S., Neugebauer, U., Schlatter, E., Houillier, P., Gahl, W. A., Vikkula, M., Bloch-Zupan, A., Bleich, M., Kitagawa, H., Unwin, R. J., Mighell, A., Berdal, A., and Kleta, R. (2013) Nephrocalcinosis (Enamel Renal Syndrome) caused by autosomal recessive FAM20A mutations. Nephron Physiol., 122, 1-6.

Nadanaka, S., Zhou, S., Kagiyama, S., Shoji, N., Sugahara, K., Sugihara, K., Asano, M., and Kitagawa, H. (2013) EXTL2, a member of EXT family of tumor suppressors, controls glycosaminoglycan biosynthesis in a xylose kinase-dependent manner. J. Biol. Chem., 288 (13), 9321-9333.

【総説】三上 雅久,北川 裕之 コンドロイチン硫酸による骨格筋分化・再生過程の制御 実験医学 Vol.31 No.10 (増刊) 2013 羊土社 52-57 

2012

Mikami, T., Koyama, S., Yabuta, Y., and Kitagawa, H. (2012)
Chondroitin sulfate is a critical determinant for skeletal muscle development/regeneration and improvement of muscular dystrophy.
J. Biol. Chem., 287 (46), 38531-38542.

Koike, T., Izumikawa, T., Tamura, J., and Kitagawa, H. (2012) Chondroitin sulfate-E fine-tunes osteoblast differentiation via ERK1/2, Smad3 and Smad1/5/8 signaling by binding to N-cadherin and cadherin-11. Biochem. Biophys. Res. Commun., 420 (3), 523-529.

Miyata, S., Komatsu, Y., Yoshimura, Y., Taya, C., and Kitagawa, H. (2012)
Persistent cortical plasticity by upregulation of chondroitin 6-sulfation.
Nature Neurosci., 15 (3), 414-422.

Tamura, J., Tsutsumishita, N., Nakao, Y., Kawano, M., Kato, S., Takeda, N., Nadanaka, S., and Kitagawa, H. (2012) Synthesis and interaction with midkine of biotinylated chondroitin sulfate tetrasaccharides. Bioorg. Med. Chem. Lett., 22 (3), 1371-1374.

Izumikawa, T., Koike, T., and Kitagawa, H. (2012) Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1. Biochem. J., 441 (2), 697-705.

2011

Miyata, S., and Kitagawa, H. (2011)
Chondroitin sulfate proteoglycans regulate experience-dependent neuronal plasticity.
Trends Glycosci. Glycotechnol., 23 (133), 239-247.

Nakagawa, N., Izumikawa, T., Kitagawa, H., and Oka, S. (2011) Sulfation of glucuronic acid in the linkage tetrasaccharide by HNK-1 sulfotransferase is an inhibitory signal for the expression of a chondroitin sulfate chain on thrombomodulin. Biochem. Biophys. Res. Commun., 415 (1), 109-113.

Anggraeni, V. Y., Emoto, N., Yagi, K., Mayasari, D. S., Nakayama, K., Izumikawa, T., Kitagawa, H., and Hirata, K. (2011) Correlation of C4ST-1 and CnGn-2 expression with chondroitin sulfate chain elongation in atherosclerosis.
Biochem. Biophys. Res. Commun., 406 (1), 36-41.

Saigoh, K., Izumikawa, T., Koike, T., Shimizu, J., Kitagawa, H., and Kusunoki, S. (2011) Chondroitin beta-1,4-N-acetylgalactosaminyltransferase-1 missense mutations are associated with neuropathies. J. Hum. Genet., 56 (2), 143-146.

Izumikawa, T., Okuura, Y., Koike, T., Sakoda, N., and Kitagawa, H. (2011) Chondroitin 4-O-sulfotransferase-1 regulates the chain length of chondroitin sulfate in cooperation with chondroitin N-acetylgalactosaminyltransferase-2. Biochem. J., 434 (2), 321-331.

Nadanaka, S., Kinouchi, H., Taniguchi-Morita, K., Tamura, J., and Kitagawa, H. (2011) Down-regulation of chondroitin 4-O-sulfotransferase-1 by Wnt signaling triggers diffusion of Wnt-3a. J. Biol. Chem., 286 (6), 4199-4208.

【著書】Koike, T., Nadanaka, S., and Kitagawa, H. (2011) Enzyme assay of xylosyltransferase. GlycoPOD: GlycoScience Protocol Online Database http://jcggdb.jp/GlycoPOD/protocolShow.action?nodeld=t84

【著書】Nadanaka, S., and Kitagawa, H. (2011) Enzyme assay of GAG glycosyltransferases for heparan sulfate. GlycoPOD: GlycoScience Protocol Online Database http://jcggdb.jp/GlycoPOD/protocolShow.action?nodeld=t83

【著書】Nadanaka, S., and Kitagawa, H. (2011) Enzyme assay of GAG glycosyltransferases for chondroitin sulfate. GlycoPOD: GlycoScience Protocol Online Database http://jcggdb.jp/GlycoPOD/protocolShow.action?nodeld=t82

【総説】宮田 真路,北川 裕之 ライフサイエンス新着論文レビュー(2011.02.07)6-硫酸化コンドロイチンの過剰発現により大脳皮質の神経可塑性が維持される http://first.lifesciencedb.jp/archives/4319

【総説】灘中 里美,北川 裕之(2011)硫酸化糖鎖による形態形成因子Wntのシグナル伝達と拡散の調節機構 生化学 第83巻 第11号 1027-1031

【著書】泉川 友美,北川 裕之「グリコサミノグリカングルクロン酸転移酵素-I」(Glucuronyltransferase-I: GlcAT-I) 出版社エル・アイ・シー『〈series モデル動物利用マニュアル〉生物機能モデルと新しいリソース・リサーチツール』(2011)375-383

【著書】北川 裕之 生物薬科学実験講座2011(廣川書店)4巻−II,pp.82-101 「1H-NMR{(2)オリゴ糖}」

【総説】三上 雅久,北川 裕之「神経系におけるコンドロイチン硫酸鎖の糖鎖暗号」生化学 第83巻 第3号(2011)231-239

【総説】宮田 真路,北川 裕之 特集 糖鎖と神経疾患 グリコサミノグリカン「コンドロイチン硫酸と神経可塑性」脳21 Vol.14 No.1 (2011) 16-21

2010
Watanabe, Y., Takeuchi, K., Higa-Onaga, S., Tsujita, M., Abe, M., Natsume R., Li, M., Furuichi, T., Saeki, M., Izumikawa, T., Hasegawa, A., Yokoyama, M., Ikegawa, S., Sakimura, K., Amizuka, N., Kitagawa, H., and Igarashi, M. (2010) Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development.
Biochem. J., 432 (1), 47-55.

Dejima, K., Murata, D., Mizuguchi, S., Nomura, K. H., Izumikawa, T., Kitagawa, H., Gengyo-Ando, K., Yoshina, S., Ichimiya, T., Nishihara, S., Mitani, S., and Nomura, K. (2010) Two Golgi-resident 3' -phosphoadenosine 5' -phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans. J. Biol. Chem., 285 (32), 24717-24728.

Izumikawa, T., and Kitagawa, H. (2010) Mice deficient in glucuronyltransferase-I. Prog. Mol. Biol. Transl. Sci. (Lijuan Zhang, ed.), 93, pp. 19-34, Elsevier.

Okada, M., Nadanaka, S., Shoji, N., Tamura, J., and Kitagawa, H. (2010) Biosynthesis of heparan sulfate in EXT-1-deficient cells. Biochem. J., 428 (3), 463-471. (Faculty of 1000)

Izumikawa, T., Kanagawa, N., Watamoto, Y., Okada, M., Saeki, M., Sakano, M., Sugahara, K., Sugihara, K., Asano, M., and Kitagawa, H. (2010) Impairment of embryonic cell division and glycosaminoglycan biosynthesis in glucuronyltransferase-I-deficient mice. J. Biol. Chem., 285 (16), 12190-12196.

 2005-2009


2009

Koike, T., Izumikawa, T., Tamura, J., and Kitagawa, H. (2009) 
FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region. 
 Biochem. J., 421 (2) 157-162.

Mizumoto, S., Mikami, T., Yasunaga, D., Kobayashi, N., Yamauchi, H., Miyake, A., Itoh, N., Kitagawa, H., and Sugahara, K. (2009) Chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish. Biochem. J. , 419 (2), 387-399.

Mikami, T., Yasunaga, D., and Kitagawa, H. (2009) 
Contactin-1 is a functional receptor for neuroregulatory chondroitin sulfate-E. J. Biol. Chem., 284 (7), 4494-4499.

【著書】北川 裕之 高速液体クロマトグラフィー{(3)アミン結合型シリカゲルカラムによる分離}生物薬科学実験講座(廣川書店)4巻−I,pp.61-73(2009)

【著書】北川 裕之 抗体カラム 生物薬科学実験講座(廣川書店)4巻−I,pp.94-115(2009)

2008

Kitagawa, H., Tsutsumi, K., Ikegami-Kuzuhara, A., Nadanaka, S., Goto, F., Ogawa, T., and Sugahara, K. (2008) 
Sulfation of the galactose residues in the glycosaminoglycan-protein linkage region by recombinant human chondroitin 6-O-sulfotransferase-1. 
 J. Biol. Chem., 283 (41), 27438-27443.

Nadanaka, S., Ishida, M., Ikegami, M., and Kitagawa, H. (2008) Chondroitin 4-O-sulfotransferase-1 modulates Wnt-3a signaling through control of E disaccharide expression of chondroitin sulfate. J. Biol. Chem., 283 (40), 27333-27343.

Mizumoto, S., and Kitagawa, H. (2008) 
Heparan sulfate synthesis and related genes. Experimental Glycoscience (Glycobiology) (Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., and Hase, S., eds.) pp. 59- 63, Springer.

Tone, Y., Pedersen, L. C., Yamamoto, T., Izumikawa, T., Kitagawa, H., Nishihara, J., Tamura, J., Negishi, M., and Sugahara, K. (2008) 2-O-phosphorylation of xylose and 6-O-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage regions synthesis. J. Biol. Chem., 283 (24), 16801-16807.

Nadanaka, S., and Kitagawa, H. (2008) Heparan Sulphate Biosynthesis and Disease. J. Biochem., 144 (1), 7-14.

Izumikawa, T., Koike T., Shiozawa, S., Sugahara, K., Tamura, J., and Kitagawa, H. (2008) Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: Chondroitin polymerization is achieved by multiple enzyme complexes consisting of chondroitin synthase family members. J. Biol. Chem., 283 (17), 11396-11406.

【総説】宮田 真路,灘中 里美,北川 裕之「受容体結合における硫酸化グリコサミノグリカンの役割」蛋白質 核酸 酵素 Vol.53 No.12 (2008)1533-1539

2007


Uyama, T., Kitagawa, H., and Sugahara, K. (2007) Biosynthesis of glycosaminoglycans and proteoglycans. Comprehensive Glycoscience (Kamerling J.P., ed.) vol. 3, pp. 79- 104, Elsevier (Amsterdam).

Purushothaman, A., Fukuda, J., Mizumoto, S., Ten Dam, G. B., van Kuppevelt, T. H., Kitagawa, H., Mikami, T., and Sugahara, K. (2007) 
Functions of Chondroitin Sulfate/Dermatan Sulfate Chains in Brain Development: CRITICAL ROLES OF E AND iE DISACCHARIDE UNITS RECOGNIZED BY A SINGLE CHAIN ANTIBODY GD3G7. J. Biol. Chem., 282 (27), 19442-19452.

Izumikawa, T., Uyama, T., Okuura, Y., Sugahara, K., and Kitagawa, H. (2007) Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin polymerizing factor. J. Biol. Chem., 403 (3), 545-552.

Kitagawa, H., Izumikawa, T., Mizuguchi, S., Dejima, K., Nomura, K. H., Egusa, N., Taniguchi, F., Tamura, J., Gengyo-Ando, K., Mitani, S., Nomura, K., and Sugahara, K. (2007) Expression of rib-1, a caenorhabditis elegans homolog of the human tumor suppressor EXT genes, is indispensable for heparan sulfate synthesis and embryonic morphogenesis. J. Biol. Chem., 282 (11), 8533-8544.

2006

Uyama, T., Ishida, M., Izumikawa, T., Trybara, E., Tufaro, F., Bergstrom, T., Sugahara, K., and Kitagawa, H. (2006) 
Chondroitin 4-O-sulfotransferase-1 regulates "E" disaccharide expression of chondroitin sulfate required for herpes simplex virus infectivity. J. Biol. Chem., 281 (50), 38668-38674.

Franks, D. M., Izumikawa, T., Kitagawa, T., Sugahara, K., and Okkema, P. G. (2006) C. elegans pharyngeal morphogenesis requires both de novo synthesis of pyrimidines and synthesis of heparan sulfate proteoglycans. J. Biol. Chem., 296 (2), 409-420.

Dejima, K., Seko, A., Yamashita, K., Gengyo-Ando, K., Mitani, S., Izumikawa, T., Kitagawa, H., Sugahara, K., Mizuguchi, S., and Nomura, K. (2006) Essential roles of 3'-phosphoadenosine 5'-phosphosulfate synthase in embryonic and larval development of the nematode Caenorhabditis elegans. J. Biol. Chem., 281 (16), 11431-11440.

Izumikawa, T., Egusa, N., Taniguchi, F., Sugahara, K., and Kitagawa, H. (2006) Heparan sulfate polymerization in Drosophila. J. Biol. Chem., 281 (4), 1929-1934.

【総説】泉川 友美,北川 裕之 Functional Glycomics News Letter 糖鎖フラッシュ号, 2006, (8) 49-58「棟転移酵素複合体によるヘパラン硫酸鎖およびコンドロイチン硫酸鎖の重合化機構」

2005

Mizumoto, S., Kitagawa, H., and Sugahara, K. (2005) 
Biosynthesis of heparin and heparan sulfate. Chemistry and Biology of Heparin and Heparan sulfate (Garg, H. G., Linhardt, R. J., and Hales, C. A., eds.), pp. 203-243, Elsevier, Oxford, UK.

Bergefall, K., Trybala, E., Johansson, M., Uyama, T., Naito, S., Yamada, S., Kitagawa, H., Sugahara, K., and Bergstrom, T. (2005) 
Chondroitin sulfate characterized by the E disaccharide unit is a potent inhibitor of herpes simplex virus infectivity and provides the virus binding sites on gro2c cells. J. Biol. Chem., 280 (37), 32193-32199.

Mizumoto, S., Uyama, T., Mikami, T., Kitagawa, H., and Sugahara, K. (2005) Biosynthetic pathways for differential expression of functional chondroitin sulfate and heparan sulfate. Handbook of Carbohydrate Engineering (Kevin J. Yarema, ed.), pp. 289-324, CRC Press (Taylor & Francis Group), Boca Raton, FL.

【著書】三上 雅久,菅原 一幸,他『糖鎖科学の新展開〜機能解明・次世代型材料・医薬品開発に向けて〜』(エヌ・ティ・エス, 2005)pp.48-54.

【著書】泉川 友美,北川 裕之,菅原 一幸,他『遺伝子医学 MOOK3 糖鎖と病気』(メディカルドゥ, 2005)pp.180-5.

【著書】 水本 秀二,北川 裕之,菅原 一幸『未来を拓く糖鎖科学』(金芳堂, 2005) pp.169-71.

 2000-2004


2004

Izumikawa, T., Kitagawa, H., Mizuguchi, S., Nomura, K. H., Nomura, K., Tamura, J., Gengyo-Ando, K., Mitani, S., and Sugahara, K. (2004) 
Nematode chondroitin polymerizing factor showing cell/organ- specific expression is indispensable for chondroitin synthesis and embryonic cell division. 
 J. Biol. Chem., 279 (51), 53755-53761.

Thiele, H., Sakano, M., Kitagawa, H., Sugahara, K., Rajab, A., Hohne, W., Leschik, G., Nurnberg, P., and Mundlos, S. (2004) Loss of chondroitin 6-O-sulfotransferase-1 function results in severe human chondrodysplasia with progressive spinal involvement. Proc. Natl. Acad. Sci. USA, 101 (27), 10155-10160.

【総説】宇山 徹, 北川 裕之, 菅原 一幸, 他 蛋白質・核酸・酵素2004,49(2),141-7. 「線虫の細胞分裂を制御する糖鎖コンドロイチン」

【総説】北川 裕之  生化学, 2004,76 (9),1175-90. 「硫酸化グリコサミノグリカンの生合成と疾患」

【総説】北川 裕之,菅原 一幸,他「実験医学」2004,22(7),921-6. 「単一細胞レベルで糖鎖の機能を探る —コンドロイチンプロテオグリカンを例として」

2003

Kim, B-T., Kitagawa, H., Tanaka, J., Tamura, J., and Sugahara, K. (2003) 
In vitro heparan sulfate polymerization: crucial roles of core protein moieties of primer substrates in addition to the EXT1-EXT2 interaction. 
 J. Biol. Chem., 278 (43), 41618-41623.

Sugahara, K., Mikami, T., Uyama, T., Mizuguchi, S., Nomura, K., and Kitagawa, H. (2003) Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate. Curr. Opin. Struct. Biol., 13, 612-620.

Mikami, T., Mizumoto, S., Kago, N., Kitagawa, H., and Sugahara K. (2003) Specificities of three distinct human chondroitin/dermatan N- acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor: implication of differential roles in dermatan sulfate biosynthesis. J. Biol. Chem., 278 (38), 36115-36127.

Mizuguchi, S., Uyama, T., Kitagawa, H., Nomura, K. H., Dejima, K., Gengyo-Ando, K., Mitani, S., Kohara, Y., Sugahara , K., and Nomura, K. (2003) Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans. Nature, 423, 443-448. (Faculty of 1000)

Kitagawa, H., Izumikawa, T., Uyama, T., and Sugahara, K. (2003) Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization. J. Biol. Chem., 278 (26), 23666-23671.

Pedersen, L.C., Dong, J., Taniguchi, F., Kitagawa, H., Krahn, J., Pedersen, L.G., Sugahara, K., and Negishi, M. (2003) Crystal structure of an a1, 4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis. J. Biol. Chem., 278 (16), 14420-14428.

Kim, B.-T., Tsuchida, K., Lincecum, J., Kitagawa, H., Bernfield, M., and Sugahara, K. (2003) Identification and characterization of three Drosophila melanogaster glucuronyltransferases responsible for the synthesis of the conserved glycosaminoglycan-protein linkage region of proteoglycans. Two novel homologs exhibit broad specificity toward oligosaccharides from proteoglycans, glycoproteins, and glycosphingolipids. J. Biol. Chem., 278 (11), 9116-9124.

Uyama, T., Kitagawa, H., Tanaka, J., Tamura, J., Ogawa, T., and Sugahara K. (2003) Molecular cloning and expression of a second chondroitin N- acetylgalactosaminyltransferase involved in the biosynthetic initiation and elongation of chondroitin/dermatan sulfate. J. Biol. Chem., 278 (5), 3072-3078.

【総説】宇山 徹,北川 裕之,菅原 一幸 蛋白質・核酸・酵素2003, 48 (8), 1019-26. 「プロテオグリカン・コア糖転移酵素」

【総説】北川 裕之, 他 蛋白質・核酸・酵素2003, 48 (8), 1057-63.「線虫を用いた糖鎖機能の網羅的解析のすすめ:プロテオグリカン関連遺伝子を例として」

【総説】北川 裕之 医学のあゆみ2003, 207 (5), 348-54.「遺伝性多発性外骨腫とヘパラン硫酸の生合成-癌抑制遺伝子EXTファミリーの解析から-」

2002

Sugahara, K., and Kitagawa, H. (2002) 
Heparin and heparan sulfate biosynthesis. 
IUBMB Life, 54, 163-175.

Kim, B.-T., Kitagawa, H., and Sugahara, K. (2002) Hereditary multiple exostoses: association with EXT family genes and defective heparan sulfate biosynthesis. Connective Tissue, 34 (3) 261-265.

Kim, B.-T., Kitagawa, H., Tamura, J., Kusche-Gullberg, M., Lindahl, U., and Sugahara, K. (2002) Demonstration of a novel gene DEXT3 of Drosophila melanogaster as the essential N-acetylglucosamine transferase in the heparan sulfate biosynthesis: chain initiation and elongation. J. Biol. Chem., 277 (16), 13659-13665.

Uyama, T., Kitagawa, H., Tamura, J., and Sugahara K. (2002) Molecular Cloning and Expression of Human Chondroitin N- Acetylgalactosaminyltransferase: The Key Enzyme for Chain Initiation and Elongation of Chondroitin/Dermatan Sulfate on the Protein Linkage Region Tetrasaccharide Shared by Heparin/Heparan Sulfate. J.  Biol. Chem., 277 (11), 8841-8846.

【総説】北川 裕之 薬学雑誌 2002, 122(7), 435-450.「生理活性糖鎖・硫酸化グリコサミノグリカンの生合成機構の解析」

2001

Kitagawa, H., Uyama, T., and Sugahara, K. (2001) 
Molecular cloning and expression of a human chondroitin synthase. 
J. Biol. Chem., 276 (42), 38721-38726.

Kitagawa, H., Taoka, M., Tone, Y., Sugahara, K. (2001) Human glycosaminoglycan glucuronyltransferase I gene and a related processed pseudogene. Genomic structure, chromosomal mapping, and characterization. Biochem. J., 358 (3), 539-546.

Kim, B.-T., Kitagawa, H., Tamura, J., Saito, T., Kusche- Gullberg, M., Lindahl, U., and Sugahara, K. (2001) The human tumor suppressor EXT gene family members, EXTL1 and EXTL3, encode alpha 1,4-N- acetylglucosaminyltransferases involved in heparan sulfate/heparin biosynthesis. Proc. Natl. Acad. Sci. USA, 98 (13), 7176-7181.

Tamura, J., Urashima, H., Tsuchida, K., Kitagawa, H., and Sugahara, K. (2001) Synthesis of linear-type chondroitin clusters having a C8 spacer between disaccharide moieties and enzymatic transfer of D-glucuronic acid to the artificial glycans. Carbohydr. Res., 322 (1), 41-51.

Kitagawa, H., Egusa, N., Tamura, J., Kusche-Gullberg, M., Lindahl, U., and Sugahara, K. (2001) rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha 1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate. J. Biol. Chem., 276 (7), 4834-4838.

【著書】Kitagawa, H., and Sugahara, K.『Glycosyltransferases and Related Genes』(Taniguchi, N., Honke, K., and Fukuda, M., eds)2001, 368-74. "GAG Glucuronyltransferase-I"

【著書】Kitagawa, H., and Sugahara, K.『Glycosyltransferases and Related Genes』(Taniguchi, N., Honke, K., and Fukuda, M., eds)2001, 385-91. "4-N-Acetylhexosaminyltransferase (EXTL2)"

【総説】北川 裕之『薬学研究の進歩』17巻, 2001, 49-59. 「新しい-N-アセチルガラクトサミン転移酵素の性質とその存在意義の解明」

【総説】菅原 一幸,山田 修平,北川 裕之『生化学』2001, 73(6), 458-70. 「硫酸化グリコサミノグリカンの生合成のメカニズム」

2000

Pedersen, L.C., Tsuchida, K., Kitagawa, H., Sugahara, K., and Negishi M. (2000) 
Heparan/chondroitin sulfate biosynthesis: structure and mechanism of human glucuronyltransferase I. 
J. Biol. Chem., 275 (44), 34580-34585.

Sugahara, K., and Kitagawa, H. (2000) 
Recent advances in the study of the biosynthesis and functions of sulfated glycosaminoglycans. Curr. Opin. Struct. Biol., 10 (5), 518-527.

Senay, C., Lind, T., Muguruma, K., Tone, Y., Kitagawa, H., Sugahara, K., Lidholt, K., Lindahl, U., and Kusche-Gullberg, M. (2000) 
Association of the EXT1 and EXT2 proteins in heparan sulfate biosynthesis. 
EMBO Reports, 1 (3), 282-286.

Kitagawa, H., Fujita, M., Ito, N., and Sugahara, K. (2000) 
Molecular cloning and expression of a novel chondroitin 6-O-sulfotransferase. 
J. Biol. Chem., 275 (28), 21075-21080.

【総説】北川 裕之,菅原 一幸『蛋白質・核酸・酵素』2000, 45(4), 31-38. 「ヘパラン硫酸の生合成と癌抑制遺伝子(EXT)ファミリー」

【総説】刀禰 裕子,北川 裕之,菅原 一幸『生化学』2000, 72(5), 373-376. 「プロテオグリカンのグリコサミノグリカンータンパク質結合領域生合成に関与するグルクロン酸転移酵素-I」

 1999 以前


1999

Yamada, S., Van Die, I., Van den Eijnden, D.H., Yokota, A., Kitagawa, H., and Sugahara, K. (1999) 
Demonstration of glycosaminoglycans in Caenorhabditis elegans. 
FEBS Letters, 459 (3), 327-331.

Tone, Y., Kitagawa, H., Oka, S., Kawasaki, T. and Sugahara, K. (1999) 
Characterization of recombinant glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans. 
FEBS Letters, 459 (3), 415-420.

Tsuchida, K., Lind, T., Kitagawa, H., Lindahl, U., Sugahara, K., and Lidholt, K. (1999) 
Purification and characterization of fetal bovine serum N-acetyl-D-galactosaminyltransferase and glucuronyltransferase involved in chondroitin sulfate biosynthesis. 
 J. Biochem., 264 (2),461-467.

Nadanaka, S., Kitagawa, H., Goto, F., Tamura, J., Neumann, K.W., Ogawa, T., and Sugahara, K. (1999) 
Involvement of the Core Protein in the First β-N-Acetylgalactosamine Transfer to the Glycosaminoglycan-Protein Linkage Region Tetrasaccharide and in the Subsequent Polymerization: The critical determining step for chondroitin sulfate biosynthesis. 
 Biochem. J., 340 (2), 353-357.

Kitagawa, H.,Shimakawa, H., and Sugahara, K.(1999) 
The Tumor Suppressor EXT-like Gene EXTL2 Encodes an α1,4-N- Acetylhexosaminyltransferase That Transfers N-Acetylgalactosamine and N-Acetylglucosamine to the Common Glycosaminoglycan-Protein Linkage Region. The key enzyme for the chain initiation of heparan sulfate. 
 J. Biol Chem., 274 (20), 13933-13937.

Kitagawa, H., Kano, Y., Shimakawa, H., Goto, F., Ogawa, T., Okabe, H. and Sugahara, K.(1999) 
Identification and characterization of a novel UDP-GalNAc: GlcAβ-R α1,4-N-acetylgalactosaminyltransferase from a human sarcoma cell line. 
Glycobiology, 9 (7), 697-703.

【総説】灘中 里美,北川 裕之,菅原 一幸 糖鎖リモデリングニュース  1999, 2(1), 5-17. 「パートタイムプロテオグリカンであるα-トロンボモジュリンに存在するO-結合型糖鎖の構造解析、及びコンドロイチン硫酸の生合成機構に関する研究」

【総説】北川 裕之,他『NEW 生化学』(富田 基郎,豊島 聰 編,廣川書店,1999)

1998

Tsutsumi, K., Shimakawa, H., Kitagawa, H., and Sugahara, K. (1998) 
Functional expression and genomic structure of human chondroitin 6-sulfotransferase. 
FEBS Letters, 441 (2), 235-241.

Nadanaka, S., Kitagawa, K. and Sugahara, K. (1998) 
Demonstration of the immature glycosaminoglycan tetrasaccharide sequence GlcAβ1-3Galβ1-3Galβ1-4Xyl on recombinant soluble human α-thrombomodulin. A possible mechanism generating "part- time" proteoglycans. 
 J. Biol Chem., 273 (50), 33728-33734.

Kitagawa, H., Tone, Y., Tamura, J., Neumann, K.W., Ogawa, T., Oka, S., Kawasaki, T., and Sugahara, K. (1998) 
Molecular cloning and expression of glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycasns. J. Biol. Chem., 273 (12), 6615-6618.

【著書】土田和徳、北川裕之、菅原一幸『糖鎖生物学 糖鎖情報発信から受信のメカニズムまで』 2594-601(共立出版、1998)「グリコサミノグリカンの糖鎖骨格の生合成」

1997

Kitagawa, H., Tsutsumi, K., Tone, Y. and Sugahara, K. (1997) 
Developmental regulation of the sulfation profile of chondroitin sulfate chains in the chicken embryo brain. J. Biol. Chem., 272 (50), 31377-31381.

Kitagawa, H., Oyama, M., Masayama, K., Yamaguchi, Y. and Sugahara, K. (1997) 
Structural variations in the glycosaminoglycan-protein linkage region of recombinant decorin expressed in chinese hamster ovary cells. 
Glycobiology, 7 (8), 1175-1180.

Kitagawa, H., Ujikawa, M., Tsutsumi, K., Tamura, J., Neumann, K., Ogawa, T. and Sugahara, K. (1997) 
Characterization of serum β-glucuronyltransferase involved in chondroitin sulfate biosynthesis. 
Glycobiology, 7 (7), 905-911.

Kitagawa, H., Tanaka, Y., Yamada, S., Seno, N., Haslam, S. M., Morris, H. R., Dell, A. and Sugahara, K. (1997) 
A novel pentasaccharide sequence GlcA(3-sulfate)(β1-3)GalNAc(4-sulfate)(β1-4)(Fucα1- 3)GlcA(β1-3)GalNAc(4-sulfate) in the oligosaccha-rides isolated from king crab cartilage chondroitin sulfate K and its differential susceptibility to chondroitinases and hyaluronidase. 
Biochemistry, 36 (13), 3998-4008.

Kitagawa, H., Tsutsumi, K., Ujikawa, M., Goto, F., Tamura, J., Neumann, K., Ogawa, T. and Sugahara, K. (1997) 
Regulation of chondroitin sulfate biosynthesis by specific sulfation: acceptor specificity of serum β-GalNAc transferase revealed by structurally-defined oligosaccharides. 
Glycobiology, 7 (4), 531-537.

Lidholt, S., Fjelstad, M., Lindahl, U., Goto, F., Ogawa, T., Kitagawa, H. and Sugahara, K. (1997) 
Assessment of glycosaminoglycan-protein linkage tetrasaccharides as acceptors for GalNAc- and GlcNAc-transferases from mouse mastocytoma. 
Glycoconjugate J., 14 (6), 737-742.

【総説】北川 裕之、菅原 一幸 Connecitive Tissue 1997, 29 (4), 267-72.「糖鎖の硫酸化によるコンドロイチン硫酸生合成の制御機構」

1996

Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R. and Dell, A. (1996) 
Novel sulfated oligosaccharides containing 3-O-sulfated glucuronic acid from king crab cartilage chondroitin sulfate K: the unexpected degradation by chondroitinase ABC. J. Biol. Chem., 271 (43), 26745-26754.

Kitagawa, H., Ujikawa, M. and Sugahara, K. (1996) 
Developmental changes in serum UDP-GlcA: chondroitin glucuronyltransferase activity. J. Biol. Chem., 271(12), 6583-6585.

【著書】北川 裕之、菅原 一幸『用語ライブラリー 細胞接着』 40-42(羊土社、1996).「ケラタン硫酸(KS)」

【著書】北川 裕之、菅原 一幸『細胞接着分子—その生体機能の全貌—』 185-191(東京化学同人、1996).「細胞外基質としての接着分子 プロテオグリカン」

1995

Kitagawa, H., Kinoshita, A. and Sugahara, K. (1995) 
Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2-aminoacridone by capillary electrophoresis and high-performance liquid chromatography. Anal. Biochem., 232 (1), 114-121.

Kitagawa, H., Tanaka, Y., Tsuchida, K., Goto, F., Ogawa, T., Lidholt, K., Lindahl., U. and Sugahara, K.(1995) 
GalNAc transfer to the common carbohydrate-protein linkage region of sulfated glycosaminoglycans: identification of UDP-GalNAc: chondro- oligosaccharide a-N-acetylgalactosaminyltransferase in bovine serum. 
 J. Biol. Chem., 270 (38), 22190-22195.

Kitagawa, H., Tsuchida, K., Ujikawa, M. and Sugahara, K. (1995) 
Detection and characterization of UDP-GalNAc: chondroitin N-acetylgalactosaminyltransferase in bovine serum using a simple assay method. 
 J. Biochem., 117 (5), 1083-1087.