Miyoshi T, Aoki Y, Uno Y, Araki M, Kamatani T, Fujii D, Fujita Y, Takeda N, Ueda M, Kitagawa H, Emoto N, Mukai T, Tanaka M, Miyata O. Michael addition-aromatization reaction of dienylimines bearing a leaving group and its application to the preparation of thiol-selective labeling reagents capable of forming strong carbon-sulfur bonds. (2013) J Org Chem. 78(22), 11433-11443.

Purnomo E, Emoto N, Nugrahaningsih DAA, Nakayama K, Yagi K, Heiden S, Nadanaka S, Kitagawa H, Hirata KI. Glycosaminoglycan overproduction in the aorta increases aortic calcification in murine chronic kidney disease. (2013) J Am Heart Assoc. 28(2), e000405.

Miyagawa K, Emoto N. A new class of drug for pulmonary arterial hypertension: Can a Rho-kinase inhibitor break the stagnation in treating it? (2013) Circ. J. 77(10), 2477-2478

Ali H, Emoto N, Yagi K, Vignon-Zellweger N, Nakayama K, Hatakeyama K, Asada Y, Rikitake Y, Hirata KI. Localization and characterization of a novel secreted protein, SCUBE2, in the development and progression of atherosclerosis. (2013) Kobe J. Med. Sci. 59(4), E122-131.

Taniguchi Y, Emoto N, Miyagawa K, Nakayama K, Kinutani H, Tanaka H, Shinke T, Okada K, Okita Y, Hirata KI. Subsequent shunt closure after targeted medical therapy can be an effective strategy for secundum atrial septal defect with severe pulmonary arterial hypertension: two case reports : Strategy for ASD with Severe PAH. (2013) Heart Vessels. 77(9), 2383-2389.

Li X, Yamagata K, Nishita M, Endo M, Arfian N, Rikitake Y, Emoto N, Hirata K, Tanaka Y, Minami Y. Activation of Wnt5a-Ror2 signaling associated with epithelial-to-mesenchymal transition of tubular epithelial cells during renal fibrosis. (2013) Genes Cells. 18, 608-619.

Taniguchi Y, Emoto N, Miyagawa K, Nakayama K, Kinutani H, Tanaka H, Shinke T, Hirata KI. Noninvasive and Simple Assessment of Cardiac Output and Pulmonary Vascular Resistance With Whole-Body Impedance Cardiography Is Useful for Monitoring Patients With Pulmonary Hypertension. (2013) Circ J. 77, 2383-2389.

Mayasari DS, Emoto N, Yagi K, Vignon-Zellweger N, Nakayama K, Miyoshi T, Miyata O, Hirata K. Rhodamine-labeled LDL as a tool to monitor the lipoprotein traffic in experimental model of early atherosclerosis in mice. (2013) Kobe J Med Sci. 59, E54-63.

Hartopo AB, Emoto N, Vignon-Zellweger N, Suzuki Y, Yagi K, Nakayama K, Hirata K. Endothelin-converting enzyme-1 gene ablation attenuates pulmonary fibrosis via CGRP-cAMP/EPAC1 pathway. (2013) Am J Respir Cell Mol Biol. 48, 465-476.

Motoji Y, Tanaka H, Fukuda Y, Ryo K, Emoto N, Kawai H, Hirata K. Efficacy of right ventricular free-wall longitudinal speckle-tracking strain for predicting long-term outcome in patients with pulmonary hypertension. (2013) Circ J. 77, 756-763.



Toh R, Ishida T, Nishimura K, Nonaka H, Inoue Y, Kitagawa Y, Suematsu M, Miki T, Emoto N, Hirata K. Comparison of medium-dose losartan/hydrochlorothiazide and maximal-dose angiotensin II receptor blockers in the treatment of Japanese patients with uncontrolled hypertension: the Kobe-CONNECT Study. (2012) Hypertens Res. 2012 Nov;35(11):1080-6.

Nishio R, Tanaka H, Tsuboi Y, Kinutani H, Taniguchi Y, Shigeru M, Toh R, Miura Y, Sakai Y, Emoto N, Kawai H, Hirata K. Differences in hemodynamic parameters and exercise capacity between patients with pulmonary arterial hypertension and chronic heart failure. (2012) J Cardiopulm Rehabil Prev. 32, 379-385.

Barton M, Emoto N, Miyauchi T. The First Tomoh Masaki Award (2012). Life Sci. 91, 466-469.

Tawaramoto K, Kotani K, Hashiramoto M, Kanda Y, Nagare T, Sakaue H, Ogawa W, Emoto N, Yanagisawa M, Noda T, Kasuga M, Kaku K. Ablation of 3-phosphoinositide-dependent protein kinase 1 (PDK1) in vascular endothelial cells enhances insulin sensitivity by reducing visceral fat and suppressing angiogenesis. (2012) Mol Endocrinol. 26, 95-109.

Satomi-Kobayashi S, Kinugasa M, Kobayashi R, Hatakeyama K, Kurogane Y, Ishida T, Emoto N, Asada Y, Takai Y, Hirata K, Rikitake Y. Osteoblast-like Differentiation of Cultured Human Coronary Artery Smooth Muscle Cells by Bone Morphogenetic Protein Endothelial Cell Precursor-derived Regulator (BMPER). (2012) J Biol Chem. 287, 30336-30345.

Nakayama K, Emoto N, Suzuki Y, Vignon-Zellweger N, Yagi K, Hirata K. Physiological relevance of hydrolysis of atrial natriuretic Peptide by endothelin-converting enzyme-1. (2012) Kobe J Med Sci. 58, E12-8.

Vignon-Zellweger N, Heiden S, Miyauchi T, Emoto N. Endothelin and endothelin receptors in the renal and cardiovascular systems. (2012) Life Sci. 91, 490-500.

Adiarto S, Heiden S, Vignon-Zellweger N, Nakayama K, Yagi K, Yanagisawa M, Emoto N. ET-1 from endothelial cells is required for complete angiotensin II-induced cardiac fibrosis and hypertrophy. (2012) Life Sci. 91, 651-657.

Arfian N, Emoto N, Vignon-Zellweger N, Nakayama K, Yagi K, Hirata K. ET-1 deletion from endothelial cells protects the kidney during the extension phase of ischemia/reperfusion injury. (2012) Biochem Biophys Res Commun. 425, 443-449.



Vignon-Zellweger N, Heiden S, Emoto N. Renal function and blood pressure: molecular insights into the biology of endothelin-1. (2011) Contrib Nephrol. 172, 18-34.

Fukuda Y, Tanaka H, Sugiyama D, Ryo K, Onishi T, Fukuya H, Nogami M, Ohno Y, Emoto N, Kawai H, Hirata K. Utility of right ventricular free wall speckle-tracking strain for evaluation of right ventricular performance in patients with pulmonary hypertension. (2011) J Am Soc Echocardiogr. 24, 1101-1108.

Anggraeni VY, Emoto N, Yagi K, Mayasari DS, Nakayama K, Izumikawa T, Kitagawa H, Hirata K. Correlation of C4ST-1 and ChGn-2 expression with chondroitin sulfate chain elongation in atherosclerosis. (2011) Biochem Biophys Res Commun. 406, 36-41.



Widyantoro B, Emoto N, Nakayama K, Anggrahini DW, Adiarto S, Iwasa N, Yagi K, Miyagawa K, Rikitake Y, Suzuki T, Kisanuki YY, Yanagisawa M, Hirata KI. Endothelial Cell-Derived Endothelin-1 Promotes Cardiac Fibrosis in Diabetic Hearts through Stimulation of Endothelial-to-Mesenchymal Transition. (2010) Circulation 121, 2407-2418.

Kisanuki YY, Emoto N, Ohuchi T, Widyantoro B, Yagi K, Nakayama K, Kedzierski RM, Hammer RE, Yanagisawa H, Williams SC, Richardson JA, Suzuki T, Yanagisawa M. Low blood pressure in endothelial cell-specific endothelin-1 knockout mice. (2010) Hypertension 56, 121-128.

Miyagawa K, Emoto N, Widyantoro B, Nakayama K, Yagi K, Rikitake Y, Suzuki T, Hirata KI. Attenuation of Doxorubicin-Induced Cardiomyopathy by ECE-1 ablation through Prevention of Mitochondrial Biogenesis Impairment. (2010) Hypertension 55, 738-746

Yagita K, Yamanaka I, Emoto N, Kawakami K, Shimada S. Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy. (2010) BMC Biotechnology 10, 3.

Doi M, Takahashi Y, Komatsu R, Yamazaki F, Yamada H, Haraguchi S, Emoto N, Okuno Y, Tsujimoto G, van der Horst GTJ, Okamura H. Salt-sensitive hypertension in circadian clock–deficient Cry-null mice involves dysregulated adrenal Hsd3b6. (2010) Nature Med. 16, 67-74.

Iwasa N, Emoto N, Widyantoro B, Miyagawa K, Hirata KI. Knockout of Endothelin-1 in Vascular Endothelial Cells Protects Against Insulin Resistance Induced by High-Salt Diet in Mice. (2010) Kobe J. Med. Sci. 56:E85- E91



Anggrahini DW, Emoto N, Nakayama K, Widyantoro B, Adiarto S, Iwasa N, Nonaka H, Rikitake Y, Kisanuki YY, Yanagisawa M, Hirata KI. Vascular endothelial cell-derived endothelin-1 mediates vascular inflammation and neointima formation following blood flow cessation. (2009) Cardiovasc Res. 82, 143-151.

Masuda Y, Emoto N, Nonaka H, Yagita K, Todo T, Okamura H, Yokoyama M, Hirata KI. Role of angiotensin and the clock system in the circadian regulation of plasminogen activator inhibitor-1. (2009) Kobe J. Med. Sci. 6, 264-271.

Nishinaga H, Komatsu R, Doi M, Fustin JM, Yamada H, Okura R, Yamaguchi Y, Matsuo M, Emoto N, Okamura H. Circadian expression of the Na+/H+ exchanger NHE3 in the mouse renal medulla. (2009) Biomed Res. 30, 87-93.



Sasaki N, Yamashita T, Takaya T, Shinohara M, Shiraki R, Takeda M, Emoto N, Fukatsu A, Hayashi T, Ikemoto K, Nomura T, Yokoyama M, Hirata KI, Kawashima S. Augmentation of Vascular Remodeling by Uncoupled Endothelial Nitric Oxide Synthase in a Mouse Model of Diabetes Mellitus. (2008) Arterioscler Thromb Vasc Biol. 28, 1068-1076.


Adiarto S, Emoto N, Iwasa N, Yokoyama M. Obesity-induced upregulation of myocardial endothelin-1 expression is mediated by leptin. (2007) Biochem Biophys Res Commun. 353:623-627.

Fukuya H, Emoto N, Nonaka H, Yagita K, Okamura H, Yokoyama M. Circadian expression of clock genes in human peripheral leukocytes. (2007) Biochem Biophys Res Commun. 354, 924-928.

Shinohara M, Hirata KI, Yamashita T, Takaya T, Sasaki N, Shiraki R, Ueyama T, Emoto N, Inoue N, Yokoyama M, Kawashima S. Local Overexpression of Toll-Like Receptors at the Vessel Wall Induces Atherosclerotic Lesion Formation. Synergism of TLR2 and TLR4. (2007) Arterioscler Thromb Vasc Biol. 27, 2384-2391.



Maekawa K, Tsujino T, Saito K, Kim JI, Ikeda Y, Emoto N, Yokoyama M. Inhibitory effect of insulin on vasopressin-induced intracellular calcium response is blunted in hyperinsulinemic hypertensive patients: role of membrane fatty acid composition. (2006) Heart Vessels. 21, 205-12.


Emoto N, Raharjo SB, Isaka D, Masuda S, Adiarto S, Jeng AY, Yokoyama M. (2005) Dual ECE/NEP Inhibition on Cardiac and Neurohumoral Function during the Transition from Hypertrophy to Heart Failure in Rats. Hypertension. 45, 1145-1152.

Rohman MS, Emoto N, Nonaka H, Okura|| R, Nishimura M, Yagita K, van der Horst GTJ, Matsuo M, Okamura H, Yokoyama M. (2005) Circadian Clock Genes Directly Regulate Expression of the Na+/H+ Exchanger NHE3 in the Kidney. Kidney International 67, 1410-1419.


Harada H, Tsujino T, Watari Y, Nonaka H, Emoto N, Yokoyama M. (2004) Oral taurine supplementation prevents fructose-induced hypertension in rats. Heart Vessels. 19, 132-136.



Ikeda K, Emoto N, Matsuo M, Yokoyama M. (2003) Molecular identification and characterization of a novel nuclear protein whose expression is up-regulated in insulin-resistant animals. J. Biol. Chem. 278, 3514-3520.

Isaka D., Emoto N., Raharjo, S. B., Yokoyama M., Matsuo M. (2003) The effects of phosphoramidon on the expression of human endothelin-converting enzyme-1 (ECE-1) isoforms. J. Cardiovascular Pharmacology 42, 136-141.

Mohri T., Emoto N., Nonaka, H., Fukuya H., Yagita K., Okamura H., Yokoyama M. (2003) Alterations of circadian expressions of clock genes in Dahl salt-sensitive rats fed a high-salt diet. Hypertension 42, 189-194.

Misawa K., Horiba T., Arimura N., Hirano Y., Inoue J., Emoto N., Shimano H., Shimizu M., Sato R. (2003) Sterol regulatory element-binding protein-2 interacts with hepatocyte nuclear factor-4 to enhance sterol isomerase gene expression in hepatocytes. J. Biol. Chem. 278, 36176-36182.

Nurhantari Y, Emoto N, Rahayu P, Matsuo M. (2003) Nasopharyngeal carcinoma in Indonesia has a low prevalence of the 30-base pair deletion of Epstein-Barr virus latent membrane protein 1. Southeast Asian J Trop Med Public Health 34, 98-105.

Rohman MS, Emoto N, Takeshima Y, Yokoyama M, Matsuo M. (2003) Decreased mAKAP, ryanodine receptor, and SERCA2a gene expression in mdx hearts. Biochem Biophys Res Commun. 310, 228-235.



Ikeda S, Emoto N, Alimsardjono H, Yokoyama M, Matsuo M. (2002) Molecular isolation and characterization of novel four subisoforms of ECE-2. Biochem. Biophys. Res. Commun. 293, 421-426.

Kulathila, R., Clark, K., Savage, P., Bowen, BR., Emoto, N., Yanagisawa, M., Jeng, AY. (2002) Expression, purification and characterization of the monomeric and dimeric forms of soluble bovine endothelin converting enzyme-1a. Clin. Sci. (Lond), 103, 94S-97S.

Hayashi T, Tsujino T, Iwata S, Nonaka H, Emoto N, Yano Y, Otani S, Hayashi Y, Itoh H, Yokoyama M. (2002) Decreased ornithine decarboxylase activity in the kidneys of Dahl salt-sensitive rats. Hypertens. Res. 25, 787-795.


Raharjo, S. B., Emoto, N., Ikeda, K., Sato, R., Yokoyama, M., and Matsuo, M. (2001). Alternative splicing regulates the endoplasmic reticulum localization or secretion of soluble secreted endopeptidase. J. Biol. Chem. 276, 25612-25620.

Nonaka, H., Tsujino, T., Watari, Y., Emoto, N, and Yokoyama, M. (2001) Taurine prevents the decrease in expression and secretion of extracellular superoxide dismutase induced by homocysteine: Amelioration of homocysteine-induced endoplasmic reticulum stress by taurine. Circulation 104, 1165-1170.

Nonaka, H., Emoto N., Ikeda K., Fukuya, H., Saifur R. M., Raharjo, S. B., Yagita, K., Okamura, H., and Yokoyama, M. (2001) Angiotensin II induces circadian gene expression of clock genes in cultured vascular smooth muscle cells. Circulation 104, 1746-1748.



Sato, R., Okamoto, A., Inoue, J., Miyamoto, W., Sakai, Y., Emoto, N., Shimano, H. and Maeda, M. (2000). Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins J. Biol. Chem. 275, 12497-12502.

Yanagisawa, H., Hammer, R. E., Richardson, J. A., Emoto, N., Williams, S. C., Takeda, S., Clouthier, D. E., and Yanagisawa, M. (2000). Disruption of ECE-1 and ECE-2 reveals a role for endothelin-converting enzyme-2 in murine cardiac development. J. Clin. Invest. 105, 1373-1382.

Seo, B., Ikeda, K., Emoto, N., Choi, D. J., Hwang, J. Y., Matsuo, M., Kim, E. J., and Cheon, I. S. (2000). Estrogen affects vascular tone differently according to vasoactive substances in ovariectomized Sprague-Dawley rat. Yonsei Med. J. 41, 49-55.


Emoto, N., Nurhantari, Y., Alimsardjono, H., Xie, J., Yamada, T., Yanagisawa, M., and Matsuo, M. (1999). Constitutive lysosomal targeting and degradation of bovine endothelin-converting enzyme-1a mediated by novel signals in its alternatively spliced cytoplasmic tail. J. Biol. Chem. 274, 1509-1518.

Ikeda, K., Emoto, N., Raharjo, S. B., Nurhantari, Y., Saiki, K., Yokoyama, M., and Matsuo M. (1999). Molecular identification and characterization of a novel membrane-bound metalloprotease, the soluble secreted form of which hydrolyzes a variety of vasoactive peptides. J. Biol. Chem. 274, 32469-32477.



Yanagisawa, H., Yanagisawa, M., Kapur, R. P., Richardson, J. A., Williams, S. C., Clouthier, D. E., de Wit, D., Emoto, N., and Hammer, R. E. (1998). Dual genetic pathways of endothelin-mediated intercellular signaling revealed by targeted disruption of endothelin converting enzyme-1 gene. Development 125, 825-836.

Telemaque, S., Emoto, N., deWit, D., and Yanagisawa, M. (1998). In vivo role of endothelin-converting enzyme-1 as examined by adenovirus-mediated overexpression in rats. J. Cardiovasc. Pharmacol. 31, S548-550.

Ahn, K., Sisneros, A. M., Herman, S. B., Pan, S. M., Hupe, D., Lee, C., Nikam, S., Cheng, X. M., Doherty, A. M., Schroeder, R. L., Haleen, S. J., Kaw, S., Emoto, N., and Yanagisawa, M. (1998). Novel selective quinazoline inhibitors of endothelin converting enzyme-1. Biochem. Biophys. Res. Commun. 243, 184-190.


Matsuura, A., Kawashima, S., Yamochi, W., Hirata, K., Yamaguchi, T., Emoto, N., and Yokoyama, M. (1997). Vascular endothelial growth factor increases endothelin-converting enzyme expression in vascular endothelial cells. Biochem. Biophys. Res. Commun. 235, 713-716.

Pupilli, C., Romagnani, P., Lasagni, L., Bellini, F., Misciglia, N., Emoto, N., Yanagisawa, M., Rizzo, M., Mannelli, M., and Serio, M. (1997). Localization of endothelin-converting enzyme-1 in human kidney. Am. J. Physiol. 273, F749-756.

Matsuura, A., Kawashima, S., Yamochi, W., Hirata, K., Yamaguchi, T., Emoto, N., and Yokoyama, M. (1997). Vascular endothelial growth factor increases endothelin-converting enzyme expression in vascular endothelial cells. Biochem. Biophys. Res. Commun. 235, 713-716.

Furukawa, K., Saleh, D., Bayan, F., Emoto, N., Kaw, S., Yanagisawa, M., and Giaid, A. (1996). Co-expression of endothelin-1 and endothelin-converting enzyme-1 in patients with chronic rhinitis. Am. J. Respir. Cell Mol. Biol. 14, 248-253.

Emoto, N., and Yanagisawa, M. (1995). Endothelin-converting enzyme-2 is a membrane-bound, phosphoramidon-sensitive metalloprotease with acidic pH optimum. J. Biol. Chem. 270, 15262-15268.

Baynash, A. G., Hosoda, K., Giaid, A., Richardson, J. A., Emoto, N., Hammer, R. E., and Yanagisawa, M. (1994). Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell 79, 1277-1285.

Xu, D., Emoto, N., Giaid, A., Slaughter, C., Kaw, S., deWit, D., and Yanagisawa, M. (1994). ECE-1: a membrane-bound metalloprotease that catalyzes the proteolytic activation of big endothelin-1. Cell 78, 473-485. *The first two authors contributed equally to this work.