核酸・タンパク質の構造・機能の特性を生かした分析技術・創薬開発に関する研究

神戸薬科大学 生命分析化学研究室

業績

業績リスト

2024年

原著論文

  1. Ohgita T., Sakai K., Fukui N., Namba N., Nakano M., Kiguchi Y., Morita I., Oyama H., Yamaki K., Nagao K., Kobayashi N., Saito H.,
    “Generation of novel anti-apoE monoclonal antibodies that selectively recognize apoE isoforms”
    FEBS letters, 598(8), 902-914 (2024). doi: 10.1002/1873-3468.14858.

  2. Kamiya Y., Lao S., Ariyoshi J., Sato F., Asanuma H.,
    “Unexpectedly stable homopurine parallel triplex of SNA:RNA*SNA and L-aTNA:RNA*L-aTNA”
    Chem. Commun., 60, 1257-1260 (2024)
    Selected as a "Front Cover"

学会発表

  1. ISBC2024 (2024年4月24-26日, Toyoda Auditorium, Nagoya University)
    [ポスター発表]
    “Development of anti-miR-21 oligonucleotide composed of SNA and artificial nucleobases”
    *Yukiko Kamiya, Fuminori Sato, Hiroyuki Asanuma

    “The Effects of Incorporating Acyclic Nucleic Acids on the Toxicity and Efficacy of Gapmer-ASOs”
    *Jumpei Ariyoshi, Ayaka Minamikawa, Mioka Okuno, Nozomi Tanigawa, Aki Tsumura, Yuuka Suzumura, Tomoki Onabe, Masaya Higuchi, Hiroyuki Asanuma, Yukiko Kamiya

  2. 日本薬学会第144年会 (2024年3月29日, パシフィコ横浜)
    [ポスター発表]
    “アンチセンス核酸に対するマウス抗体の創製”
    *大山浩之, 大和璃音, 田中寿理, 有吉純平, 神谷由紀子

    [口頭発表]
    “Gapmerアンチセンス核酸の毒性調節における非環状型人工核酸の効果”
    *有吉純平, 樋口昌也, 今村早希, 大塚彩乃, 奧野未央佳, 谷川望, 南川綾香, 大山浩之, 浅沼浩之, 神谷由紀子

  3. 日本化学会第104春季年会 (2024年3月18-21日, 日本大学理学部 船橋キャンパス)
    [口頭発表]
    “非環状型人工核酸導入によるGapmer型アンチセンス核酸の毒性変化”
    *有吉 純平, 樋口 昌也, 浅沼 浩之, 神谷 由紀子

    “非環状型人工核酸からなる抗miR-21核酸の開発”
    *神谷 由紀子, 佐藤 史経, 浅沼 浩之

2023年

原著論文

  1. Zhu H., Kamiya Y., Asanuma H.
    "Illuminating miRNA inhibition: Visualizing the interaction between anti-miRNA oligonucleotide and target miRNA using FRET"
    ACS Chem. Biol., 18, 2281-2289 (2023). DOI: doi.org/10.1021/acschembio.3c00353
    Selected as a "Front Cover"

  2. Ohgita T., Kono H., Morita I., Oyama H., Shimanouchi T., Kobayashi N., Saito H.
    "Intramolecular interaction kinetically regulates fibril formation by human and mouse α-synuclein"
    Sci. Rep., 13, 10885 (2023).

2023年3月以前

神谷 由紀子

原著論文

  1. Sato, F.; Kamiya, Y.*; Asanuma, H.*, “Syntheses of Base-Labile Pseudo-Complementary SNA and L-aTNA Phosphoramidite Monomers”, J. Org. Chem., 2023, 88, 2, 796–804
  2. Kamiya, Y.*; Kamimoto, H.; Zhu, H.; Asanuma, H.*, “Development of pre-miRNAs modification with FRET dye pair for intracellular visualization of processing intermediates generated in cells”, Sensors, 2021, 21, 1785. IF:3.576
  3. Kamiya, Y.*; Satoh, T.; Kodama, A.; Suzuki, T.; Murayama, K.; Kashida, H.; Uchiyama, S.; Kato, K.; Asanuma, H.*, “Intrastrand backbone-nucleobase interactions stabilize unwound right-handed helical structures of heteroduplexes of L-aTNA/RNA and SNA/RNA”, Commun. Chem., 2020, 3, 156. IF:6.581
  4. Kamiya, Y.*; Takeyama, Y.; Mizuno, T.; Sato, F.; Asanuma, H.*, “Investigation of strand selective interaction of SNA-modified siRNA with AGO2-MID”, Int. J. Mol. Sci., 2020, 21, 5218. IF:5.924
  5. Yagi, H.; Yagi-Utsumi, M.; Honda, R.; Ohta, Y.; Saito, T.; Nishio, M.; Ninagawa, S.; Suzuki, K.; Anzai, T.; Kamiya, Y.; Aoki, K.; Nakanishi, M.; Satoh, T.; Kato, K.*, “Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor”, Nat. Commun., 2020, 11, 1368. IF:14.919
  6. Satoh, T.; Nishio, M.; Suzuki, K.; Yagi-Utsumi, M.; Kamiya, Y.; Mizushima, T.; Kato, K.*, “Crystallographic snapshots of the EF-hand protein MCFD2 complexed with the intracellular lectin ERGIC-53 involved in glycoprotein transport”, Acta Crystallogr. F, 2020, 76, 216-221. IF:0.568
  7. Chen, Y.; Murayama, K.; Kashida, H.; Kamiya, Y.; Asanuma, H.*, “A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity”, Chem. Commun., 2020, 56, 5358-5361. IF:6.222
  8. Kamiya, Y.*; Sato, F.; Murayama, K.; Kodama, A.; Uchiyama, S.; Asanuma, H.*, “SNA/RNA hybridization promoted with 2,6-diaminopurine and 2-thiouracil, which suppress self-hybridization of SNA”, Chem. Asian J., 2020, 15, 1266-1271, Selected as a “Cover Picture” IF: 4.568
  9. Kashida, H.; Hattori, Y.; Tazoe, K.; Inoue, T.; Nishikawa, K.; Ishii, K.; Uchiyama, S.; Yamashita, H.; Abe, M.; Kamiya, Y.; Asanuma, H.*, “Bifacial nucleobases for hexaplex formation in aqueous solution”, J. Am. Chem. Soc., 2018, 140, 8456-8462. IF:15.419
  10. Kamiya, Y.*; Arimura, Y.; Ooi, H.; Kato, K.; Liang, X.G.; Asanuma, H.*, “Development of visible light-responsive RNA scissors based on the 10-23 DNAzyme”, ChemBioChem, 2018, 19, 1305-1311. IF:3.164
  11. Kamiya, Y.*; Yamada, Y.; Muro, T.; Matsuura, K.; Asanuma, H.*, “DNA microcapsule for photo-triggered drug release system”, ChemMedChem, 2017, 12, 2016-2021. Selected as a “Front Cover” IF:3.466
  12. Kamiya, Y*.; Donoshita, Y.; Kamimoto, H.; Murayama, K.; Ariyoshi, J.; Asanuma, H.*, “Introduction of 2,6-diaminopurines into serinol nucleic acid (SNA) improves anti-miRNA performance”, ChemBioChem, 2017, 18, 1917-1922. IF:3.164
  13. Nakasone, Y.; Ooi, H.; Kamiya, Y.; Asanuma, H.; Terazima, M.*, “Dynamics of Inter-DNA Chain Interaction of Photoresponsive DNA”, J. Am. Chem. Soc., 2016, 138, 9001-9004.
  14. Aasanuma, H.*; Niwa, R.; Akahane, M.; Murayama, K.; Kashida, H.; Kamiya, Y., “Strand-invading linear probe combined with unmodified PNA”, Bioorg. Med. Chem., 2016, 24, 4129-4137.
  15. Kanematsu, Y.; Kamiya, Y.; Matsuo, K.; Gekko, K.; Kato, K.; Tachikawa, M.*, “Isotope effect on the circular dichroism spectrum of methyl α-D-glucopyranoside in aqueous solution”, Sci. Rep., 2015, 5, 17900,
  16. Kamiya, Y.; Iishiba, K.; Doi. T.; Tsuda, K.; Kashida, H.; Asanuma, H.*, “Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2”, Biomater. Sci., 2015, 3, 1534-1538, Selected as a “Back cover”
  17. Ninagawa, S.; Okada, T.; Sumitomo, Y.; Horimoto, S.; Sugimoto, T.; Ishikawa, T.; Takeda, S.; Yamamoto, T.; Suzuki, T.; Kamiya, Y.; Kato, K.; Mori, K.*, “Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway”, J. Cell Biol., 2015, 211, 775-784
  18. Inagaki, K.; Satoh, T.; Yagi-Utsumi, M.; Gulluche, A.C.; Anzai, T.; Uekusa, Y.; Kamiya, Y.; Kato, K.*, “Redox-coupled structural changes of the catalytic a’ domain of protein disulfide isomerase”, FEBS Lett., 2015, 589, 2690-2694
  19. Murayama, K.; Kamiya, Y.; Kashida, H.; Asanuma, H.*, “Ultra-Sensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cell”, ChemBioChem, 2015, 16, 1298-1301
  20. Kashida, H.; Osawa, T.; Morimoto, K.; Kamiya, Y.; Asanuma, H.*, “Molecular design of Cy3 derivative for highly sensitive in-stem molecular beacon and its application to the wash-free FISH”, Bioorg. Med. Chem., 2015, 23, 1758-1762
  21. Asanuma, H.*; Akahane, M.; Niwa, R.; Kashida, H.; Kamiya, Y., “Highly sensitive and robust linear probe for detection of mRNA in cells”, Angew. Chem. Int. Ed., 2015, 54, 4315-4319
  22. Kamiya, Y.; Takagi, T.; Ooi, H.; Ito, H.; Liang, X.G.; Asanuma, H.*, “Synthetic gene involving azobenzene-tethered T7 promoter for the photocontrol of gene expression by visible light.”, ACS Synth. Biol., 2015, 4, 365-370.
  23. Kamiya, Y.; Takai, J.; Ito, H.; Murayama, K.; Kashida, H.;Asanuma, H.*, “Enhancement of stability and activity of siRNA by terminal substitution with Serinol Nucleic Acid (SNA).”, ChemBioChem, 2014, 15, 2549-2555., Selected as a “Back Cover”
  24. Ninagawa, S.; Okada, T.; Sumitomo, Y.; Kamiya, Y.; Kato, K.; Horimoto, S.; Ishikawa, T.; Takeda, S.; Sakuma, T.; Yamamoto, T.; Mori, K.*, “EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step”, J. Cell Biol., 2014, 206, 347-356.
  25. Kamiya, Y.; Ito, A.; Ito, H.; Urushihara, M.; Takai, J.; Fujii, T.; Liang, X.G.; Kashida, H.; Asanuma, H.*, “Selective labeling of mature RISC using siRNA carrying fluorophore-quencher pair”, Chem. Sci., 2013, 4, 4016-4021.
  26. Araki,K.; Iemura, S.; Kamiya, Y.; Ron, D.; Kato, K.; Natsume, T.; and Nagata, K.*, “Ero1-α and PDIs constitute a hierarchical electron transfer network of endoplasmic reticulum oxidoreductases”, J. Cell Biol., 2013, 202, 861-874.
  27. Horimoto, S.; Ninagawa, S.; Okada, T,; Koba, H.; Sugimoto, T.; Kamiya, Y.; Kato, K.; Takeda, S.; and Mori, K.* “The unfolded protein response transducer ATF6 represents a novel transmembrane-type endoplasmic reticulum-associated degradation substrate requiring both mannose trimming and SEL1L protein”, J. Biol. Chem., 2013, 288, 31517-27.
  28. Kumoi, K.; Satoh, T.; Murata, K.; Hiromoto, T.; Mizushima, T.; Kamiya, Y.; Noda, M.; Uchiyama, S.; Yagi, H.; Kato, K.*, “An archaeal homolog of proteasome assembly factor functions as a proteasome activator”, PLoS ONE, 2013, 8, e60294.
  29. Yamaguchi, T.; Kamiya, Y.; Choo, Y.M.; Yamamoto, S.; Kato, K.*, “Terminal Spin Labeling of a High-mannose-type Oligosaccharide for Quantitative NMR Analysis of Its Dynamic Conformation”, Chem. Lett., 2013, 42, 544-546.
  30. Fujimori, T.; Kamiya, Y.; Nagata, K.; Kato, K.; Hosokawa, N.*, “Endoplasmic reticulum lectin XTP3-B inhibits endoplasmic reticulum-associated degradation of a misfolded α1-antitrypsin variant”, FEBS J., 2013, 280, 1563-1575.
  31. Kamiya, Y.; Yanagi, K.; Kitajima, T.; Yamaguchi, T.; Chiba, Y.; Kato, K.*, “Application of metabolic 13C labeling in conjunction with high-field NMR spectroscopy for comparative conformational analysis of high mannose-type oligosaccharides”, Biomolecules, 2013, 3, 108-123.
  32. Kondo, S.; Yagi, H.; Kamiya, Y.; Ito, A.; Kuhara, M.; Kudoh, A.; Takahashi, N.; Kato, K.*, “N-Glycosylation Profiles of Chicken Immunoglobulin Y Glycoproteins Expressed by Different Production Vehicles”, J. Glycomics and Lipidomics, 2012, S5. 002.
  33. Kamiya, Y.; Uekusa, Y.; Sumiyoshi, A.; Sasakawa, H.; Hirao, T.; Suzuki, T.; Kato, K.*, “NMR characterization of the interaction between the PUB domain of peptide:N-glycanase and ubiquitin-like domain of HR23″, FEBS Lett., 2012, 586, 1141-1146.
  34. Kamiya, Y.; Yamamoto, S.; Chiba, Y.; Jigami, Y.; Kato, K.*, “Overexpression of a homogeneous oligosaccharide with 13C labeling by genetically engineered yeast strain”, J. Biomol. NMR, 2011, 50, 397-401.
  35. Hosokawa, N.; Tremblay, L.O.; Sleno, B.; Kamiya, Y.; Wada, I.; Nagata, K.; Kato, K.; Herscovics, A.*, “EDEM1 accelerates the trimming of α1,2-linked mannose on the C branch of N-glycans”, Glycobiology, 2010, 20, 567-575.
  36. Nishio, M.; Kamiya, Y.; Mizushima, T.; Wakatsuki, S.; Sasakawa, H.; Yamamoto, K.; Uchiyama, S.; Noda, M.; McKay, A.R.; Fukui, K.; Hauri, H.-P.; Kato, K.*, “Structural basis for the cooperative interplay between the two causative gene products of combined factor V and factor VIII deficiency”, Proc. Natl. Acad. Sci. USA, 2010, 107, 4034-4039.
  37. Serve, O.+; Kamiya, Y.+; Maeno, A.+; Nakano, M.+; Murakami, C.; Sasakawa, H.; Yamaguchi, Y.; Harada, T.; Kurimoto, E.; Yagi-Utsumi, M.; Iguchi, T.; Inaba, K.; Kikuchi, J.; Asami, O.; Kajino, T.; Oka, T.; Nakasako, M.; Kato, K.*(+These authors contributed equally to this work), “Redox-dependent domain rearrangement of protein disulfide isomerase coupled with exposure of its substrate-binding hydrophobic surface”, J. Mol. Biol., 2010, 396, 361-374.
  38. Dan, H.; Kamiya, Y.; Totani, K.; Kamiya, D.; Kawasaki, N.; Yamaguchi, D.; Matsuo, I.; Matsumoto, N.; Ito, Y.; Kato, K.; Yamamoto, K.*, “Sugar-binding activity of the MRH domain in ER α-glucosidase II β subunit is important for efficient glucose trimming”, Glycobiology, 2009, 19, 1127-1135.
  39. Hosokawa, N.+; Kamiya, Y.+; Kamiya, D.; Kato, K. and Nagata, K.* (+Both authors contributed equally to this work), “Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans”, J. Biol. Chem., 2009, 284, 17061-17068.
  40. Quan, E.M.; Kamiya, Y.; Kamiya, D.; Denic, V.; Weibezahn, J.; Kato, K.; Weissman, J.S.*, “Defining the glycan destruction signal for endoplasmic reticulum-associated degradation”, Mol. Cell, 2008, 32, 870-877.
  41. Nyfeler, B.; Kamiya, Y.; Boehlen, F.; Yamamoto, K.; Kato, K.; Moerloose, P. de.; Hauri, H. -P.; Neerman-Arbez, M.*, “Deletion of three residues from the C-terminus of MCFD2 affects binding to ERGIC-53 and causes combined factor V and VIII deficiency”, Blood, 2008, 111, 1299-1301.
  42. Kamiya, Y.; Kamiya, D.; Yamamoto, K.; Nyfeler, B.; Hauri, H. -P.; Kato, K.*, “Molecular basis of sugar recognition by the human L-type lectins ERGIC-53, VIPL and VIP36”, J. Biol. Chem., 2008, 283, 1857-1861.
  43. Yamaguchi, Y.; Hirao, T.; Sakata, E.; Kamiya, Y.; Kurimoto, E.; Yoshida, Y.; Suzuki, T.; Tanaka, K.; Kato, K.*, “Fbs1 protects the malfolded glycoproteins from the attack of peptide:N-glycanase.”, Biochem. Biophys. Res. Commun., 2007, 362, 712-716.
  44. Kamiya, Y.; Yamaguchi, Y.; Takahashi, N.; Arata, Y.; Kasai, K.; Ihara, Y.; Matsuo, I.; Ito, Y.; Yamamoto, K.; Kato, K.*, “Sugar-binding properties of VIP36, an intracellular animal lectin operating as a cargo receptor”, J. Biol. Chem., 2005, 280, 37178-37182.

総説

  1. Asanuma, H.; Kamiya, Y.; Kashida, H.; Murayama, K., “Xeno nucleic acids (XNAs) having non-ribose scaffolds with unique supramolecular properties”, Chem. Commun., 2022, 28, 3993. IF:6.222
  2. Asanuma, H.; Murayama, K.; Kamiya, Y.; Kashida, H., “The DNA duplex as an aqueous one-dimensional soft crystal scaffold for photochemistry.”, Bull. Chem. Soc. Jpn., 2018, 91, 1739-1748. IF:5.488
  3. Asanuma, H.; Murayama, K.; Kamiya, Y.; Kashida, H., “Design of photo-functional oligonucleotide by “copolymerization” of natural nucleobases with base-surrogate prepared from acyclic scaffold.”, Polymer J., 2017, 49, 279-289. IF:3.080
  4. Kamiya, Y.; Asanuma, H., “Pre-organized guide RNA in Cas9 complex is ready for selection of the target double-stranded DNA”, ChemBioChem, 2015, 16, 2273-2275
  5. Kamiya, Y.; Asanuma, H., “Reversible photo-switching of DNA fuction with azobenzene-tethered DNA”, The Glen Report, 2015, 27, 1-3
  6. Asanuma, H.; Kashida, H.; Kamiya, Y., “De Novo Design of Functional Oligonucleotides with Acyclic Scaffolds”, Chem. Rec. 2014, 14, 1055-1069.
  7. Kamiya, Y.; Asanuma, H., “Light-driven DNA nanomachine with a photoresponsive molecular engine”, Acc. Chem. Res., 2014, 47, 1663-1672.
  8. Kamiya, Y.; Satoh T.; Kato, K., “Recent advances in glycoprotein production for structural biology:toward tailored design of glycoforms”, Curr. Opin. Struct. Biol., 2014, 26, 44-53.
  9. Kamiya, Y.; Satoh, T.; Kato, K., “Molecular and structural basis for N-glycan-dependent determination of glycoprotein fates in cells”, Biochim. Biophys. Acta – Gen. Subj., 2012, S5, 1327-1337.
  10. Kamiya, Y.; Yagi-Utsumi, M.; Yagi, H.; Kato, K., “Structural and molecular basis of carbohydrate-protein interaction systems as potential therapeutic targets”, Curr. Pharm. Des., 2011, 17, 1672-1684.
  11. Hosokawa, N.; Kato, K.; Kamiya, Y., “Mannose 6-phosphate receptor homology domain-containing lectins in mammalian endoplasmic reticulum-associated degradation”, Methods Enzymol., 2010, 480, 181-197.
  12. Hosokawa, N.; Kamiya, Y.; Kato, K., “The role of MRH domain-containing lectins in ERAD”, Glycobiology, 2010, 20, 651-660.
  13. 神谷由紀子、加藤晃一, “糖鎖によるタンパク質社会の秩序維持”, 化学工業, 2010, 61, 23-31.
  14. 神谷由紀子、加藤晃一, “糖鎖認識を介した糖タンパク質の細胞内運命の決定機構”, 生物物理, 2009, 49, 62-69.
  15. 神谷由紀子、加藤晃一, “細胞内レクチンによる糖蛋白質の輸送と品質管理の構造基盤”, 蛋白質　核酸　酵素, 2008, 53, 1662-1669.
  16. Kato, K.; Sasakawa, H.; Kamiya, Y.; Utsumi, M.; Nakano, M.; Takahashi, N.; Yamaguchi, Y., “920 MHz ultra-high field NMR approaches to structural glycobiology”, Biochim. Biophys. Acta – Gen. Subj., 2008, 1780, 619-625.
  17. Kato, K.; Kamiya, Y., “Structural views of glycoprotein-fate determination in cells.”, Glycobiology, 2007, 17, 1031-1044.
  18. Kamiya, Y.; Kato, K., “Sugar recognition by intracellular lectins that determine the fates of glycoproteins.”, Trends Glycosci. Glycotech, 2006, 18, 231-244.

著書

  1. 浅沼浩之, 神谷由紀子, 光応答性DNAの搭載によるナノマシンとナノ構造体の光制御, “進化を続ける核酸化学”　日本化学会編, p53-58, 化学同人 (2021)
  2. 神谷由紀子, 医薬品開発における中分子領域(核酸医薬・ペプチド医薬)の開発戦略, （担当： 分担執筆）株式会社 情報機構 (2019)
  3. 浅沼浩之、神谷由紀子, “ナノマテリアルとしての光応答性DNA”, “刺激応答性高分子ハンドブック”、監修：宮田　隆志、p.581-587、エヌ・ティー・エス (2018)
  4. 神谷由紀子、村山恵司、樫田 啓、浅沼浩之, “非環状骨格型人工核酸：aTNA, SNA”, “核酸医薬の創製と応用展開”、監修：和田 猛、p79-86、シーエムシー出版 (2016)
  5. Zhang, Y.; Yamaguchi, T.; Yagi-Utsumi, M.; Kamiya, Y.; Sakae, Y.; Okamoto, Y.; Kato, K., Conformational dynamics of oligosaccharides characterized by paramagnetism-assisted NMR spectroscopy in conjunction with molecular dynamics simulation, Springer (Switzerland) (2014)
  6. 神谷由紀子、浅沼浩之, “核酸医薬を目指した機能性siRNA”, 最先端メディカルエンジニアリング 第２版, p94-98 (2014)
  7. 神谷由紀子、浅沼浩之, “ナノ環境に優しい光応答性DNAの設計”, “太陽エネルギー社会を築く材料テクノロジー（I）　-材料・デバイス編-” 名古屋大学大学院工学研究科 材料バックキャストテクノロジー研究センター　編, p192-210コロナ社 (2013)
  8. O. Serve, Y. Kamiya, and K. Kato, Redox-Dependent Chaperoning, Following PDI Footsteps, NOVA Science Publishers, (2010)
  9. Y. Kamiya, D. Kamiya, R. Urade, T. Suzuki, and K. Kato, Sophisticated Modes of Sugar Recognition by Intracellular Lectins Involved in Quality Control of Glycoproteins, NOVA Science Publishers, (2009)

大山 浩之

原著論文

  1. Su J., Zhu B., Inoue A., Oyama H., Morita I., Dong J., Yasuda T., Sugita-Konishi Y., Kitaguchi T., Kobayashi N., Miyake S., Ueda H.
    The Patrol Yeast: A new biosensor armed with antibody-receptor chimera detecting a range of cell wall-impermeable toxic substances associated with food poisoning
    Biosens. Bioelectron., 219, 114793 (2023).
  2. Yamaki K., Ohta K., Kobayashi N., Morita I., Kiguchi Y., Oyama H., Ito K., Nanbo A., Oh-oka H., Koyama Y., Kawata Y., Fujisawa H., Ohta M.
    Purification of Emu IgY for therapeutic and diagnostic use based on monoclonal secondary antibodies specific to Emu IgY.
    Biol Pharm Bull., 45(8), 1022-1026 (2022).
  3. Morita I., Kiguchi Y., Nakamura S., Yoshida A., Kubo H., Ishida M., Oyama H., Kobayashi N.
    More than 370-fold increase in antibody affinity to estadiol-17β by exploring substitutions in the VH-CDR3.
    Biol. Pharm. Bull., 45(7), 851-855 (2022).
  4. Morita I., Kiguchi Y., Oyama H., Yamaki K., Sakio N., Kashiwabara K., Kuroda Y., Ito A, Yokota A., Ikeda N., Kikura-Hanajiri R., Ueda H., Numazawa S., Yoshida T., Kobayashi N.
    Derivatization-assisted immunoassay: application for group-specific detection of potent methamphetamine and amphetamine enantiomers.
    Anal. Methods, 14, 2745-2753 (2022).
  5. Morita I., Kiguchi Y., Oyama H., Takeuchi A., Tode C., Tanaka R. Ogata J., Kikura-Hanajiri R., Kobayashi N.
    Derivatization-assisted enzyme-linked immunosorbent assay for identifying hallucinogenic mushrooms with enhanced sensitivity.
    Anal. Methods, 13, 3954–3962 (2021).
  6. Kiguchi Y., Oyama H., Morita I., Nagata Y., Umezawa N., Kobayashi N.
    The VH framework region 1 as a target of efficient mutagenesis for generating a variety of affinity-matured scFv mutants.
    Sci. Rep., 11, 8201 (2021).
  7. Oyama H., Kiguchi Y., Morita I., Miyashita T., Ichimura A., Miyaoka H., Izumi A., Terasawa S., Osumi N., Tanaka H., Niwa T., Kobayashi N.
    NanoLuc luciferase as a suitable fusion partner of recombinant antibody fragments for developing sensitive luminescent immunoassays.
    Anal. Chim. Acta, 1161, 238180 (2021).
  8. Ohgita T., Furutani Y., Nakano M., Hattori M., Suzuki A., Nakagawa M., Naniwa S., Morita I., Oyama H., Nishitsuji K., Kobayashi N., Saito H.
    Novel conformation-selective monoclonal antibodies against apoA-I amyloid fibrils.
    FEBS J., 288, 1496-1513 (2021).
  9. Kiguchi Y., Oyama H., Morita I., Morikawa M., Nakano A., Fujihara W., Inoue Y., Sasaki M., Saijo Y., Kanemoto Y., Murayama K., Baba Y., Takeuchi A., Kobayashi N.
    Clonal array profiling of scFv-displaying phages for high-throughput discovery of affinity-matured antibody mutants.
    Sci. Rep., 10, 14103 (2020).
  10. Morita I., Oyama H., Kiguchi Y., Oguri A., Fujimoto N., Takeuchi A., Tanaka R. Ogata J., Kikura-Hanajiri R., Kobayashi N.
    Immunochemical monitoring of psilocybin and psilocin to identify hallucinogenic mushrooms.
    Pharm. Biomed. Anal., 190, 113485 (2020).
  11. Dong J., Miyake C., Yasuda T., Oyama H., Morita I., Tsukahara T., Takahashi M., Jeong H.-J., Kitaguchi T., Kobayashi N., Ueda H.
    PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor.
    Biosens. Bioelectron., 165, 112425 (2020).
  12. Oyama H., Kiguchi Y., Morita I., Yamamoto C., Higashi Y., Taguchi M., Tagawa T., Enami Y., Takamine Y., Hasegawa H., Takeuchi A., Kobayashi N.
    Seeking high-priority mutations enabling successful antibody-breeding: systematic analysis of a mutant that gained over 100-fold enhanced affinity.
    Sci. Rep., 10, 4807 (2020).
  13. Kiguchi Y., Oyama H., Morita I., Katayama E., Fujita M., Narasaki M., Yokoyama A., Kobayashi N.
    Antibodies and engineered antibody fragments against M13 filamentous phage to facilitate phage-display-based molecular breeding.
    Biol. Pharm. Bull., 41(7), 1062-1070 (2018).
  14. Morita I., Oyama H., Kanda Y., Yasuo M., Ito A., Toyota M., Hayashi Y., Yokoyama T., Kobayashi N.
    Enantioselective monoclonal antibodies for detecting ketamine to crack down on illicit use.
    Biol. Pharm. Bull., 41(1), 123-131 (2018).
  15. Oyama H., Morita I., Kiguchi Y., Morishita T., Fukushima S., Nishimori Y., Niwa T., Kobayashi N.
    A single-step “breeding” generated a diagnostic anti-cortisol antibody fragment with over 30-fold enhanced affinity.
    Biol. Pharm. Bull., 40(12), 2191-2198 (2017).
  16. Kimura H., Mikawa S., Mizuguchi C., Horie Y., Morita I., Oyama H., Ohgita T., Nishitsuji K., Takeuchi A., Lund-Katz S., Akaji K., Kobayashi N., Saito H.
    Immunochemical approach for monitoring of structural transition of ApoA-I upon HDL formation using novel monoclonal antibodies.
    Sci. Rep., 7, 2988 (2017).
  17. Morita I., Oyama H., Yasuo M., Matsuda K., Katagi K., Ito A., Tatsuda H., Tanaka H., Morimoto S., Kobayashi N.
    Antibody fragments for on-site testing of cannabinoids generated via in vitro affinity maturation.
    Biol. Pharm. Bull., 40(2), 174-181 (2017).
  18. Oyama H., Morita I., Kiguchi Y., Banzono E., Ishii K., Kubo S., Watanabe Y., Hirai A., Kaede C., Ohta M., Kobayashi N.
    One-shot in vitro evolution generated an antibody fragment for testing urinary cotinine with more than 40-fold enhanced affinity.
    Anal. Chem., 89(1), 988-995 (2017).
  19. Oyama H., Morita I., Kiguchi Y., Miyake S., Moriuchi A., Akisada T., Niwa T., Kobayashi N.
    Gaussia luciferase as a genetic fusion partner with antibody fragments for sensitive immunoassay monitoring of clinical biomarkers.
    Anal. Chem., 87(24), 12387-12395 (2015).
  20. Oyama H., Tanaka E., Kawanaka T., Morita I., Niwa T., Kobayashi N.
    Anti-idiotype scFv-enzyme fusion proteins: A clonable analyte-mimicking probe for standardized immunoassays targeting small biomarkers.
    Anal. Chem., 85(23), 11553-11559 (2013).
  21. Oyama H., Yamaguchi S., Nakata S., Niwa T., Kobayashi N.
    “Breeding” diagnostic antibodies for higher assay performance: A 250-fold affinity-matured antibody mutant targeting a small biomarker.
    Anal. Chem., 85(10), 4930-4937 (2013).
  22. Kobayashi N., Odaka K., Uehara T., Imanaka-Yoshida K., Kato Y., Oyama H., Tadokoro H., Akizawa H., Tanada S., Hiroe M., Fukumura T., Komuro I., Arano Y., Yoshida T., Irie T.
    Toward in vivo imaging of heart disease using a radiolabeled single-chain Fv fragment targeting tenascin-C.
    Anal. Chem., 83(23), 9123-9130 (2011).
  23. Kobayashi N., Banzono E., Shimoda Y., Oyama H., Kunihiro T., Morita I., Ohta M.
    A monoclonal antibody-based enzyme-linked immunosorbent assay for human urinary cotinine to monitor tobacco smoke exposure.
    Anal. Methods, 3(9), 1995-2002 (2011).
  24. Kobayashi N., Oyama H., Suzuki I., Kato Y., Umemura T., Goto J.
    Oligosaccharide-assisted direct immunosensing of small molecules.
    Anal. Chem., 82(11), 4333-4336 (2010).
  25. Kobayashi N., Oyama H., Kato Y., Goto J., Söderlind E., Borrebaeck C. A. K.
    Two-step in vitro antibody affinity maturation enables estradiol-17β assays with more than 10-fold higher sensitivity.
    Anal. Chem., 82(3), 1027-1038 (2010).
  26. Niwa T., Kobayashi T., Sun P., Goto J., Oyama H., Kobayashi N.
    An enzyme-linked immunometric assay for cortisol based on idiotype-anti-idiotype reactions.
    Anal. Chim. Acta, 638(1), 94-100 (2009).
  27. Kobayashi N., Oyama H., Nakano M., Kanda T., Banzono E., Kato Y., Karibe T., Nishio T., Goto J.
    “Cleavable” hapten-biotin conjugates: preparation and use for the generation of anti-steroid single-domain antibody fragments.
    Anal. Biochem., 387(2), 257-266 (2009).
  28. Kobayashi N., Kato Y., Oyama H., Taga S., Niwa T., Sun P., Ohtoyo M., Goto J.
    Anti-estradiol-17β single-chain Fv fragments: generation, characterization, gene randomization, and optimized phage display.
    Steroids, 73(14), 1485-1499 (2008).

総説

  1. Kobayashi N. and Oyama H.
    Antibody engineering toward high-sensitivity high-throughput immunosensing of small molecules.
    Analyst, 136(4), 642-651 (2011).
  2. 加藤芳徳, 大山浩之, 小林典裕, 後藤順一.
    低分子化抗体フラグメント―バイオメディカル分析化学における新しい分子認識単位―
    臨床化学, 36(2), 125-139 (2007).
  3. 小林典裕, 加藤芳徳, 大山浩之, 後藤順一.
    抗体工学を基盤とする超高感度ハプテンイムノメトリックアッセイへのアプローチ
    YAKUGAKU ZASSHI, 127(1), 55-69 (2007).

著書

  1. 小林典裕, 大山浩之, 森田いずみ.
    6.3 抗体の試験管内親和性成熟, p152-158,「免疫測定法～基礎から先端まで」生物化学的測定研究会編 講談社, 東京, (2014).
  2. 小林典裕, 大山浩之.
    3.3 低分子抗原に対する抗体の調製, p68-80,「免疫測定法～基礎から先端まで」生物化学的測定研究会編 講談社, 東京, (2014).
  3. 小林典裕, 大山浩之.
    2.1 抗体の構造と機能, p22-33,「免疫測定法～基礎から先端まで」生物化学的測定研究会編 講談社, 東京, (2014).

トピックス

  1. 大山浩之.
    「体外イメージングを指向したRFPレッドシフト変異体の効率的創製」
    ファルマシア, 47(10), 942-943 (2011).

有吉 純平

原著論文

  1. Yamayoshi A, Miyoshi D, Zouzumi YK, Matsuyama Y, Ariyoshi J, Shimada N, Murakami A, Wada T, Maruyama A. Selective and Robust Stabilization of Triplex DNA Structures Using Cationic Comb-type Copolymers. J Phys Chem B. (16):4015-4022. (2017)
  2. J. Ariyoshi, Y. Matsuyama, A. Kobori, A. Murakami, H. Sugiyama, A. Yamayoshi Effective anti-miRNA oligonucleotides show high releasing rate of microRNA from RNA-induced silencing complex (RISC) Nucleic Acid Ther., 27, 303-308 (2017)
  3. J. Ariyoshi, N. Eimori, A. Kobori, A. Murakami, H. Sugiyama, A. Yamayoshi Characterization of the releasing profile of microRNA from RISC using anti-miRNA oligonucleotides. Chem. Lett., 46, 143-145 (2017)
  4. J. Ariyoshi, D. Momokawa, N. Eimori, A. Kobori, A. Murakami, A. Yamayoshi Development of Novel Antisense Oligonucleotides for the Functional Regulation of RNA-Induced Silencing Complex (RISC) by Promoting the Release of microRNA from RISC.
    Bioconjugate Chem., 26, 2454-2460 (2015)
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