biochem_05
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Publication list

INDEX
2016 (16/06/08)
2015 2014
2013 2012
2011 2010
2009 2008
2007 2006
2005 2004
2003 2002
2001 2000
1999 1998
1997 1996
1995





2016 (16/06/08)
  • Takase, H., Tanaka, M., Yamamoto, A., Watanabe, S., Takahashi, S., Nadanaka, S., Kitagawa, H., Yamada, T., and Mukai, T. (2016)
    Structural requirements of glycosaminoglycans for facilitating_amyloid fibril formation of human serum amyloid A.
    Amyloid, 23(2) 67-75.

  • Miyata, S., and Kitagawa, H. (2016)
    Chondroitin sulfate and neuronal disorders.
    Front. Biosci., 21(7) 1330-1340.

  • Saigoh, K., Yoshimura, S., Izumikawa, T., Miyata, S., Tabara, Y., Matsushita, T., Miki, T., Miyamoto, K., Hirano, M., Kitagawa, H., Kita, J., and Kusunoki, S. (2016)
    Chondroitin sulfate β-1,4-N-acetylgalactosaminyltransferase-1 (ChGn-1) polymorphism; association with progression of multiple sclerosis.
    Neurosci. Res., 108, 55-59.

  • Miyata, S., and Kitagawa, H. (2016)
    Chondroitin 6-sulfation regulates perineuronal net formation by controlling the stability of aggrecan.
    Neural Plast., 2016, 1305801, 13 pages.

  • Kinouchi, H., Matsuyama, K., Kitagawa, H., and Kamimori, H. (2016)
    Surface Plasmon Resonance Assay of Inhibition by Pharmaceuticals for Thyroxine Hormone Binging to Transport Proteins.
    Anal. Biochem., 492, 43-48.





2015
  • Yabuno, K., Morise, J., Kizuka, Y., Hashii, N., Kawasaki N., Takahashi, S., Miyata, S., Izumikawa, T., Kitagawa, H., Takematsu, H., and Oka, S. (2015)
    A sulfated glycosaminoglycan linkage region is a novel type of Human Natural Killer-1 (HNK-1) epitope expressed on aggrecan in perineuronal nets.
    PLoS ONE, 10(12), e0144560.

  • Yutsudo, N., and Kitagawa, H. (2015)
    Involvement of chondroitin 6-sulfation in temporal lobe epilepsy.
    Exp. Neurol., 274 PartB, 126-133.

  • Capurro, M., Shi, W., Izumikawa, T., Kitagawa, H., and Filmus, J. (2015)
    Processing by Convertases Is Required for Glypican-3-induced Inhibition of Hedgehog Signaling.
    J. Biol. Chem., 290(12) 7576-7585.

  • Koike, T., Mikami, T., Shida, M., Habuchi, O., and Kitagawa, H. (2015)
    Chondroitin sulfate-E mediates estrogen-induced osteoanabolism.
    Sci. Rep., 5, 8994.

  • Izumikawa, T., and Kitagawa, H. (2015)
    Amino acid sequence surrounding the chondroitin sulfate attachment site of thrombomodulin regulates chondroitin polymerization.
    Biochem. Biophys. Res. Commun., 460(2) 233-237.

  • Tomatsu, S., Shimada, T., Patel, P., Mason, R. W., Mikami, T., Kitagawa, H., Montano, A. M., and Orii, T. (2015)
    Chondroitin and keratan sulfate.
    Sulfated Polysaccharides (Gama, M., Nader, H., and de Oliveira Rocha, H., eds) pp17-71, Nova Science Publishers.

  • Izumikawa, T., Sato, B., Mikami, T., Tamura, J., Igarashi, M., and Kitagawa, H. (2015)
    GlcUAβ1-3Galβ1-3Galβ1-4Xyl(2-O-phosphate) is the preferred substrate for chondroitin N-acetylgalactosaminyltransferase-1.
    J. Biol. Chem., 290(9) 5438-5448.

  • Mikami, T., and Kitagawa, H. (2015)
    Glycan structure and neural plasticity.
    Sugar chains (Taniguchi, N., Suzuki, T., and Ohtsubo, K., eds) pp107-126, Springer.

  • Taniguchi, M., Nadanaka, S., Tanakura, S., Sawaguchi, S., Midori, S., Kawai, Y., Yamaguchi, S., Shimada, Y., Nakamura, Y., Matsumura, Y., Fujita, N., Araki, N., Yamamoto, M., Oku, M., Wakabayashi, S., Kitagawa, H., and Yoshida, H. (2015)
    TFE3 is a bHLH-ZIP-type transcription factor that regulates the mammalian Golgi stress response.
    Cell Struct. Funct., 40(1) 13-30.

  • Miyata, S., and Kitagawa, H. (2015)
    Mechanisms for modulation of neural plasticity and axon regeneration by chondroitin sulfate.
    J. Biochem., 157(1) 13-22.





2014
  • Kitagawa, H. (2014)
    Using sugar-remodeling to study chondroitin sulfate function.
    Biol. Pharm. Bull., 37(11) 1705-1712.

  • Kinouchi, H., Arimoto, H., Nishiguchi, K., Oka, M., Maki, H., Kitagawa, H., and Kamimori, H. (2014)
    Binding Properties of Antimicrobial Agents to Lipid Membranes Using Surface Plasmon Resonance.
    Biol. Pharm. Bull., 37(8) 1383-1389.

  • Nadanaka, S., and Kitagawa, H. (2014)
    EXTL2 controls liver regeneration and aortic calcification through xylose kinase-dependent regulation of glycosaminoglycan biosynthesis.
    Matrix Biol., 35, 18-24.

  • Nadanaka, S., Purunomo, E., Takeda, N., Tamura, J., and Kitagawa, H. (2014)
    Heparan Sulfate Containing Unsubstituted Glucosamine Residues: Biosynthesis and Heparanase Inhibitory Activity.
    J. Biol. Chem., 289(22), 15231-15243.

  • Mikami, T., and Kitagawa, H. (2014)
    Glycosaminoglycans: their modes of action for a possible new avenue for therapeutic intervention.
    Glycoscience: Biology and Medicine (Taniguchi, N., Endo, T., Hart, G.W., Seeberger, P.H., and Wong, C.H., eds) pp511-517, Springer.

  • Miyamoto, K., Tanaka, N., Moriguchi, K., Ueno, R., Kadomatsu, K., Kitagawa, H., and Kusunoki, S. (2014)
    Chondroitin 6-O-sulfate ameliorates experimental autoimmune encephalomyelitis.
    Glycobiology, 24(5), 469-475.

  • Nadanaka, S., and Kitagawa, H. (2014)
    Beta-1,3-glucuronyltransferase 3 (glucuronyltransferase I) (B3GAT3).
    Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp849-861, Springer.

  • Nadanaka, S., and Kitagawa, H. (2014)
    Exostosin (multiple)-like 1-3 (EXTL1-3).
    Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp885-903, Springer.

  • Nadanaka, S., and Kitagawa, H. (2014)
    Exostosin 1 and 2 (EXT1, 2).
    Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp905-923, Springer.

  • Nadanaka, S., and Kitagawa, H. (2014)
    Chondroitin polymerizing factor, chondroitin polymerizing factor 2, chondroitin sulfate synthase 1, 3 (CHPF, CHPF2, CHSY1, CHSY3).
    Handbook of Glycosyltransferases and Related Genes, 2nd Ed (Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., and Angata, T., eds) pp.947-963, Springer.

  • Kinouchi, H., Arimoto, H., Nishiguchi, K., Oka, M., Maki, H., Kitagawa, H., and Kamimori, H. (2014)
    Binding properties of antimicrobial agents to dipeptide terminal of lipid II using surface Plasmon resonance.
    Anal. Biochem., 452, 67-75.

  • Koike, T., Izumikawa, T., Sato, B., and Kitagawa, H. (2014)
    Identification of phosphatase that dephosphorylates xylose in the glycosaminoglycan-protein linkage region of proteoglycans.
    J. Biol. Chem., 289(10), 6695-6708.

  • Izumikawa, T., Sato, B., and Kitagawa, H. (2014)
    Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells.
    Sci. Rep., 4, 3701.





2013
  • Takeuchi, K., Yoshioka, N., Higa Onaga, S., Watanabe, Y., Miyata, S., Wada, Y., Kudo, C., Okada, M., Ohko, K., Oda, K., Sato, T., Yokoyama, M., Matsushita, N., Nakamura, M., Okano, H., Sakimura, K., Kawano, H., Kitagawa, H., and Igarashi, M. (2013)
    Chondroitin sulphate N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury.
    Nat. Commun. 4, 2740.

  • Miyoshi, T., Aoki, Y., Uno, Y., Araki, M., Kamatani, T., Fujii, D., Fujita, Y., Takeda, N., Ueda, M., Kitagawa, H., Emoto, N., Mukai, T., Tanaka, M., and Miyata, O. (2013)
    Michael Addition-Aromatization Reaction of Dienylimines Bearing a Leaving Group and its Application to the Preparation of Thiol Selective Labeling Reagents Capable of Forming Strong Carbon-Sulfur Bonds.
    J. Org. Chem., 78(22), 11433-11443.

  • Purunomo, E., Emoto, N., Nugrahaningsih, D., Nakayama, K., Yagi, K., Heiden, S., Nadanaka, S., Kitagawa, H., and Hirata, K. (2013)
    Glycosaminoglycan overproduction in the aorta increases aortic calcification in murine chronic kidney disease.
    J. Am. Heart Assoc., 2(5), e000405.

  • Izumikawa, T., Saigoh, K., Shimizu, J., Tsuji, S., Kusunoki, S., and Kitagawa, H. (2013)
    A chondroitin synthase-1 (ChSy-1) missense mutation in a patient with neuropathy impairs the elongation of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1.
    Biochim. Biophys. Acta, 1830(10), 4806-4812.

  • Mikami, T., and Kitagawa, H. (2013)
    Biosynthesis and function of chondroitin sulfate.
    Biochim. Biophys. Acta, 1830(10), 4719-4733.

  • Nadanaka, S., Kagiyama, S., and Kitagawa, H. (2013)
    Roles of EXTL2, a member of EXT family of tumor suppressors, in liver injury and regeneration processes.
    Biochem. J., 454(1), 133-145.

  • Jaureguiberry, G., De la Dure-Molla, M., Parry, D., Quentric, M., Himmerkus, N., Koike, T., Poulter, J., Klootwijk, E., Robinette, S. L., Howie, A. J., Patel, V., Figueres, M. -L., Stanescu, H. C., Issler, N, Nicholson, J. K., Bockenhauer, D., Laing, C., Walsh, S. B., McCredie, D. A., Povey, S., Asselin, A, Picard, A., Coulomb, A., Medlar, A. J., Bailleul-Forestier, I., Verloes, A,. Le Caignec, C., Roussey, G., Guiol, J., Isidor, B., Logan, C., Shore, R., Johnson, C., Inglehearn, C., Al-Bahlani, S., Schmittbuhl, M., Clauss, F., Huckert, M., Laugel, V., Ginglinger, E., Pajarola, S., Sparta, G., Bartholdi, D., Rauch, A., Addor, M. -C., Yamaguti, P. M., Safatle, H. P., Acevedo, A. C., Martelli-Junior, H., dos Santos Netos, P. E., Coletta, R. D., Gruessel, S., Sandmann, C., Ruehmann, D., Langman, C. B., Scheinman, S. J., Ozdemir-Ozenen, D., Hart, T. C., Hart, P. S., Neugebauer, U., Schlatter, E., Houillier, P., Gahl, W. A., Vikkula, M., Bloch-Zupan, A., Bleich, M., Kitagawa, H., Unwin, R. J., Mighell, A., Berdal, A., and Kleta, R. (2013)
    Nephrocalcinosis (Enamel Renal Syndrome) caused by autosomal recessive FAM20A mutations.
    Nephron. Physiol., 122, 1-6.

  • Nadanaka, S., Zhou, S., Kagiyama, S., Shoji, N., Sugahara, K., Sugihara, K., Asano, M., and Kitagawa, H. (2013)
    EXTL2, a member of EXT family of tumor suppressors, controls glycosaminoglycan biosynthesis in a xylose kinase-dependent manner.
    J. Biol. Chem., 288(13), 9321-9333.





2012
  • Mikami, T., Koyama, S., Yabuta, Y., and Kitagawa, H. (2012)
    Chondroitin sulfate is a critical determinant for skeletal muscle development/regeneration and improvement of muscular dystrophy.
    J. Biol. Chem., 287(46), 38531-38542.

  • Koike, T., Izumikawa, T., Tamura, J., and Kitagawa, H. (2012)
    Chondroitin sulfate-E fine-tunes osteoblast differentiation via ERK1/2, Smad3 and Smad1/5/8 signaling by binding to N-cadherin and cadherin-11.
    Biochem. Biophys. Res. Commun., 420(3), 523-529.

  • Miyata, S., Komatsu, Y., Yoshimura, Y., Taya, C., and Kitagawa, H. (2012)
    Persistent cortical plasticity by upregulation of chondroitin 6-sulfation.
    Nat. Neurosci., 15(3), 414-422.

  • Tamura, J., Tsutsumishita, N., Nakao, Y., Kawano, M., Kato, S., Takeda, N., Nadanaka, S., and Kitagawa, H. (2012)
    Synthesis and interaction with midkine of biotinylated chondroitin sulfate tetrasaccharides.
    Bioorg. Med. Chem. Lett., 22(3), 1371-1374.

  • Izumikawa, T., Koike, T., and Kitagawa, H. (2012)
    Chondroitin 4-O-sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N-acetylgalactosaminyltransferase-1.
    Biochem. J., 441(2), 697-705.





2011
  • Miyata, S., and Kitagawa, H. (2011)
    Chondroitin sulfate proteoglycans regulate experience-dependent neuronal plasticity.
    Trends Glycosci. Glycotechnol., 23(133), 239-247.

  • Nakagawa, N., Izumikawa, T., Kitagawa, H., and Oka, S. (2011)
    Sulfation of glucuronic acid in the linkage tetrasaccharide by HNK-1 sulfotransferase is an inhibitory signal for the expression of a chondroitin sulfate chain on thrombomodulin.
    Biochem. Biophys. Res. Commun., 415(1), 109-113.

  • Anggraeni, V. Y., Emoto, N., Yagi, K., Mayasari, D. S., Nakayama, K., Izumikawa, T., Kitagawa, H., and Hirata, K. (2011)
    Correlation of C4ST-1 and CnGn-2 expression with chondroitin sulfate chain elongation in atherosclerosis.
    Biochem. Biophys. Res. Commun., 406(1), 36-41.

  • Saigoh, K., Izumikawa, T., Koike, T., Shimizu, J., Kitagawa, H., and Kusunoki, S. (2011)
    Chondroitin beta-1,4-N-acetylgalactosaminyltransferase-1 missense mutations are associated with neuropathies.
    J. Hum. Genet., 56(2), 143-146.

  • Izumikawa, T., Okuura, Y., Koike, T., Sakoda, N., and Kitagawa, H. (2011)
    Chondroitin 4-O-sulfotransferase-1 regulates the chain length of chondroitin sulfate in cooperation with chondroitin N-acetylgalactosaminyltransferase-2.
    Biochem. J., 434(2), 321-331.

  • Nadanaka, S., Kinouchi, H., Taniguchi-Morita, K., Tamura, J., and Kitagawa, H. (2011)
    Down-regulation of chondroitin 4-O-sulfotransferase-1 by Wnt signaling triggers diffusion of Wnt-3a.
    J. Biol. Chem., 286(6), 4199-4208.

目 TOP
2010
  • Watanabe, Y., Takeuchi, K., Higa-Onaga, S., Tsujita, M., Abe, M., Natsume R., Li, M., Furuichi, T., Saeki, M., Izumikawa, T., Hasegawa, A., Yokoyama, M., Ikegawa, S., Sakimura, K., Amizuka, N., Kitagawa, H., and Igarashi, M. (2010)
    Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development.
    Biochem. J., 432(1), 47-55.

  • Dejima, K., Murata, D., Mizuguchi, S., Nomura, K. H., Izumikawa, T., Kitagawa, H., Gengyo-Ando, K., Yoshina, S., Ichimiya, T., Nishihara, S., Mitani, S., and Nomura, K. (2010)
    Two Golgi-resident 3' -phosphoadenosine 5' -phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans.
    J. Biol. Chem., 285(32), 24717-24728.

  • Izumikawa, T., and Kitagawa, H. (2010)
    Mice deficient in glucuronyltransferase-I.
    in Prog. Mol. Biol. Transl. Sci. (Lijuan Zhang, ed.), 93, 19-34, Elsevier.

  • Okada, M., Nadanaka, S., Shoji, N., Tamura, J., and Kitagawa, H. (2010)
    Biosynthesis of heparan sulfate in EXT-1-deficient cells.
    Biochem. J., 428(3), 463-471.

  • Izumikawa, T., Kanagawa, N., Watamoto, Y., Okada, M., Saeki, M., Sakano, M., Sugahara, K., Sugihara, K., Asano, M., and Kitagawa, H. (2010)
    Impairment of embryonic cell division and glycosaminoglycan biosynthesis in glucuronyltransferase-I-deficient mice.
    J. Biol. Chem., 285(16), 12190-12196.

目 TOP
2009
  • Koike, T., Izumikawa, T., Tamura, J., and Kitagawa, H. (2009)
    FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region.
    Biochem. J., 421(2) 157-162.

  • Mizumoto, S., Mikami, T., Yasunaga, D., Kobayashi, N., Yamauchi, H., Miyake, A., Itoh, N., Kitagawa, H., and Sugahara, K. (2009)
    Chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish.
    Biochem. J., 419(2), 387-399.

  • Mikami, T., Yasunaga, D., and Kitagawa, H. (2009)
    Contactin-1 is a functional receptor for neuroregulatory chondroitin sulfate-E.
    J. Biol. Chem., 284(7), 4494-4499.

目 TOP
2008
  • Kitagawa, H., Tsutsumi, K., Ikegami-Kuzuhara, A., Nadanaka, S., Goto, F., Ogawa, T., and Sugahara, K. (2008)
    Sulfation of the galactose residues in the glycosaminoglycan-protein linkage region by recombinant human chondroitin 6-O-sulfotransferase-1.
    J. Biol. Chem., 283(41), 27438-27443.

  • Nadanaka, S., Ishida, M., Ikegami, M., and Kitagawa, H. (2008)
    Chondroitin 4-O-sulfotransferase-1 modulates Wnt-3a signaling through control of E disaccharide expression of chondroitin sulfate.
    J. Biol. Chem., 283(40), 27333-27343.

  • Mizumoto, S., and Kitagawa, H. (2008)
    Heparan sulfate synthesis and related genes.
    in Experimental Glycoscience (Glycobiology) (Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., and Hase, S., eds.) pp. 59- 63, Springer.

  • Tone, Y., Pedersen, L. C., Yamamoto, T., Izumikawa, T., Kitagawa, H., Nishihara, J., Tamura, J., Negishi, M., and Sugahara, K. (2008)
    2-O-phosphorylation of xylose and 6-O-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage regions synthesis.
    J. Biol. Chem., 283(24), 16801-16807.

  • Nadanaka, S., and Kitagawa, H. (2008)
    Heparan Sulphate Biosynthesis and Disease.
    J. Biochem., 144(1), 7-14.

  • Izumikawa, T., Koike T., Shiozawa, S., Sugahara, K., Tamura, J., and Kitagawa, H. (2008)
    Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: Chondroitin polymerization is achieved by multiple enzyme complexes consisting of chondroitin synthase family members.
    J. Biol. Chem., 283(17), 11396-11406.

  • Properzi, F., Lin, R., Kwok, J., Naidu, M., van Kuppevelt, T. H., Ten Dam, G. B., Camargo, L. M., Raha-Chowdhury, R., Furukawa, Y., Mikami, T., Sugahara, K., and Fawcett, J. W. (2008)
    Heparan sulphate proteoglycans in glia and in the normal and injured CNS: expression of sulphotransferases and changes in sulphation.
    Eur. J. Neurosci., 27(3), 593-604.

  • Akita, K., von Holst, A., Furukawa, Y., Mikami, T., Sugahara, K., and Faissner, A. (2008)
    Expression of Multiple Chondroitin/Dermatan Sulfotransferases in the Neurogenic Regions of the Embryonic and Adult CNS imply that complex Chondroitin Sulfates have a Role in Neural Stem Cell Maintenance.
    Stem Cells., 26(3), 798-809.

目 TOP
2007
  • Sugahara, K., and Mikami, T. (2007)
    Chondroitin/dermatan sulfate in the central nervous system.
    Curr. Opin. Struct. Biol., 17(5), 536-545.

  • Uyama, T., Kitagawa, H., and Sugahara, K. (2007)
    Biosynthesis ofglycosaminoglycans and proteoglycans.
    in Comprehensive Glycoscience (Kamerling J.P., ed.) vol. 3, pp. 79- 104, Elsevier (Amsterdam).

  • Purushothaman, A., Fukuda, J., Mizumoto, S., Ten Dam, G. B., van Kuppevelt, T. H., Kitagawa, H., Mikami, T., and Sugahara, K. (2007)
    Functions of Chondroitin Sulfate/Dermatan Sulfate Chains in Brain Development: CRITICAL ROLES OF E AND iE DISACCHARIDE UNITS RECOGNIZED BY A SINGLE CHAIN ANTIBODY GD3G7.
    J. Biol. Chem., 282(27), 19442-19452.

  • Izumikawa, T., Uyama, T., Okuura, Y., Sugahara, K., and Kitagawa, H. (2007)
    Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin polymerizing factor.
    Biochem. J., 403(3), 545-552.

  • Kitagawa, H., Izumikawa, T., Mizuguchi, S., Dejima, K., Nomura, K. H., Egusa, N., Taniguchi, F., Tamura, J., Gengyo-Ando, K., Mitani, S., Nomura, K., and Sugahara, K. (2007)
    Expression of rib-1, a caenorhabditis elegans homolog of the human tumor suppressor EXT genes, is indispensable for heparan sulfate synthesis and embryonic morphogenesis.
    J Biol Chem., 282(11), 8533-8544.

  • Deepa, S. S., Kalayanamitra, K., Ito, Y., Kongtawelert, P., Fukui, S., Yamada, S., Mikami, T., and Sugahara, K. (2007)
    Novel sulfated octa- and decasaccharides from squid cartilage chondroitin sulfate-E: Sequencing and application for determination of the epitope structure of the monoclonal antibody MO-225.
    Biochemistry, 46(9), 2453-2465.

目 TOP
2006
  • Uyama, T., Ishida, M., Izumikawa, T., Trybara, E., Tufaro, F., Bergstrom, T., Sugahara, K., and Kitagawa, H. (2006)
    Chondroitin 4-O-sulfotransferase-1 regulates "E" disaccharide expression of chondroitin sulfate required for herpes simplex virus infectivity.
    J. Biol. Chem., 281(50), 38668-38674.

  • Franks, D. M., Izumikawa, T., Kitagawa, T., Sugahara, K., and Okkema, P. G. (2006)
    C. elegans pharyngeal morphogenesis requires both de novo synthesis of pyrimidines and synthesis of heparan sulfate proteoglycans.
    Dev. Biol., 296(2), 409-420.

  • Mikami, T., and Sugahara, K. (2006)
    The biological importance of specific sulfation of chondroitin sulfate/dermatan sulfate in their functional expression.
    Trends Glycosci. Glycotechnol., 18(101), 165-183.

  • Mitsunaga, C., Mikami, T., Mizumoto, S., Fukuda, J., and Sugahara, K. (2006)
    Chondroitin sulfate/dermatan sulfate hybrid chains in the development of cerebellum: Spatiotemporal regulation of the expression of critical disulfated disaccharides by specific sulfotransferases.
    J. Biol. Chem., 281(28), 18942-18952.

  • Deepa, S. S., Carulli, D., Galtrey, C., Rhodes, K., Fukuda, J., Mikami, T., Sugahara, K., and Fawcett, J. W. (2006)
    Composition of perineuronal net extracellular matrix in rat brain: A different disaccharide composition for the net-associated proteoglycans.
    J. Biol. Chem., 281(26), 17789-17800.

  • Dejima, K., Seko, A., Yamashita, K., Gengyo-Ando, K., Mitani, S., Izumikawa, T., Kitagawa, H., Sugahara, K., Mizuguchi, S., and Nomura, K. (2006)
    Essential roles of 3'-phosphoadenosine 5'-phosphosulfate synthase in embryonic and larval development of the nematode Caenorhabditis elegans.
    J. Biol. Chem., 281(16), 11431-11440.

  • Izumikawa, T., Egusa, N., Taniguchi, F., Sugahara, K., and Kitagawa, H. (2006)
    Heparan sulfate polymerization in Drosophila.
    J. Biol. Chem., 281(4), 1929-1934.

目 TOP
2005
  • Mizumoto, S., Kitagawa, H., and Sugahara, K. (2005)
    Biosynthesis of heparin and heparan sulfate.
    in Chemistry and Biology of Heparin and Heparan sulfate (Garg, H. G., Linhardt, R. J., and Hales, C. A., eds.), pp. 203-243, Elsevier, Oxford, UK.

  • Bao, X., Muramatsu, T., and Sugahara, K. (2005)
    Demonstration of the pleiotrophin-binding oligosaccharide sequences isolated from chondroitin sulfate/dermatan sulfate hybrid chains of embryonic pig brains.
    J. Biol. Chem., 280(42), 35318-35328.

  • Bergefall, K., Trybala, E., Johansson, M., Uyama, T., Naito, S., Yamada, S., Kitagawa, H., Sugahara, K., and Bergstrom, T. (2005)
    Chondroitin sulfate characterized by the E disaccharide unit is a potent inhibitor of herpes simplex virus infectivity and provides the virus binding sites on gro2c cells.
    J. Biol. Chem., 280(37), 32193-32199.

  • Bao, X., Pavao, M. S., Dos Santos, J. C., and Sugahara, K. (2005)
    A functional dermatan sulfate epitope containing Iduronate(2-O- sulfate)alpha1-3GalNAc(6-O-sulfate) disaccharide in the mouse brain: Demonstration using a novel monoclonal antibody raised against dermatan sulfate of Ascidian ascidia nigra.
    J. Biol. Chem., 280(24), 23184-23193.

  • *Mizumoto, S., *Uyama, T., Mikami, T., Kitagawa, H., and Sugahara, K. (*contributed equally) (2005)
    Biosynthetic pathways for differential expression of functional chondroitin sulfate and heparan sulfate.
    In Handbook of Carbohydrate Engineering (Kevin J. Yarema, ed.), CRC Press (Taylor & Francis Group), Boca Raton, FL, 289-324.

  • Ito, Y., Hikino, M., Yajima, Y., Mikami, T., Sirko, L., von Holst, A., Faissner, A., Fukui, S., and Sugahara, K. (2005)
    Structural characterization of the epitopes of the monoclonal antibodies 473HD, CS-56 and MO-225 specific for chondroitin sulfate D- type using the oligosaccharide library.
    Glycobiology, 15(6), 593-603.

  • Bao, X., Mikami, T., Yamada, S., Faissner, A., Muramatsu, T., and Sugahara, K. (2005)
    Heparin-binding growth factor, pleiotrophin, mediates neuritogenic activity of embryonic pig brain-derived chondroitin sulfate/dermatan sulfate hybrid chains.
    J. Biol. Chem., 280(10), 9180-9191.

  • Properzi, F., Carulli, D., Asher, R. A., Muir, E., Camargo, L. M., van Kuppevelt, T. H., ten Dam, G. B., Furukawa, Y., Mikami, T., Sugahara, K., Toida, T., Geller, H. M., and Fawcett, J. W. (2005)
    Chondroitin 6-sulfate synthesis is induced by brain injury-related cytokines and enhanced in axon-growth inhibitory glia.
    Eur. J. Neurosci., 21(2), 378-390.

  • Nandini, C.D., Itoh, N., and Sugahara, K. (2005)
    Novel 70 kDa Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains with a Unique Heterogenous Sulfation Pattern from Shark Skin, which Exhibit Neuritogenic Activity and Binding Activities for Growth Factors and Neurotrophic Factors.
    J. Biol. Chem., 280(6), 4058-4069.

目 TOP
2004
  • Izumikawa, T., Kitagawa, H., Mizuguchi, S., Nomura, K. H., Nomura, K., Tamura, J., Gengyo-Ando, K., Mitani, S., and Sugahara, K. (2004)
    Nematode chondroitin polymerizing factor showing cell/organ- specific expression is indispensable for chondroitin synthesis and embryonic cell division.
    J. Biol. Chem., 279(51), 53755-53761.

  • Nandini, C.D., Mikami, T., Ohta, M., Itoh, N., Akiyama- Nambu, F., and Sugahara, K. (2004)
    Structural and functional characterization of oversulfated chondroitin sulfate/dermatan sulfate hybrid chains from the notochord of hagfish: neuritogenic activity and binding activities toward growth factors and neurotrophic factors.
    J. Biol. Chem., 279(49), 50799-50809.

  • Deepa, S.S., Yamada, S., Zako, M., Goldberger O., and Sugahara K. (2004)
    Chondroitin sulfate chains on syndecan-1 and syndecan-4 from normal murine mammary gland epithelial cells are structurally and functionally distinct, and co-operate with heparan sulfate chains to bind growth factors: A NOVEL FUNCTION TO CONTROL BINDING OF MIDKINE, PLEIOTROPHIN AND BASIC FIBROBLAST GROWTH FACTOR.
    J. Biol. Chem., 279(36), 37368-37376.

  • Kinoshita-Toyoda, A., Yamada, S., Haslam, S.M., Khoo, K.-H., Sugiura, M., Morris, H.R., Dell, A., and Sugahara, K. (2004)
    Structural determination of five novel tetrasaccharides containing 3-O-sulfated D-glucuronic acid and two rare oligosaccharides containing a beta-D-glucose branch isolated from squid cartilage chondroitin sulfate E.
    Biochemistry, 43(34), 11063-11074.

  • Yamada, S., Busse, M., Ueno, M., Kelly|, O.G., Skarnes, W.C., Sugahara, K., and Kusche-Gullberg M. (2004)
    Embryonic fibroblasts with a gene trap mutation in Ext1 produce short heparan sulfate chains.
    J. Biol. Chem., 279(31), 32134-32141.

  • Thiele, H., Sakano, M., Kitagawa, H., Sugahara, K., Rajab, A., Hohne, W., Leschik, G., Nurnberg, P., and Mundlos, S. (2004)
    Loss of chondroitin 6-O-sulfotransferase-1 function results in severe human chondrodysplasia with progressive spinal involvement.
    Proc. Natl. Acad. Sci. USA, 101(27), 10155-10160.

  • Bao, X., Nishimura, S., Mikami, T., Yamada, S., Itoh, N., and Sugahara, K. (2004)
    Chondroitin sulfate/dermatan sulfate hybrid chains from embryonic pig brain, which contain a higher proportion of L-iduronic acid than those from the adult pig brain, exhibit neuritogenic and growth factor-binding activities.
    J. Biol. Chem., 279(11), 9765-9776.

  • Umehara, Y., Yamada, S., Nishimura, S., Shioi, J., K. Robakis, N. K., and Sugahara, K. (2004)
    Chondroitin sulfate of appican, the proteoglycan form of amyloid precursor protein, produced by C6 glioma cells interacts with heparin- binding neuroregulatory factors.
    FEBS Letters, 557(1-3), 233-238.
目 TOP
2003
  • Hikino, M., Mikami, T., Faissner, A., Vilela-Silva, A-C. E. S., Pavao, M. S. G., and Sugahara, K. (2003)
    Oversulfated dermatan sulfate exhibits neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons: Implication of dermatan sulfate for neuritogenesis.
    J. Biol. Chem., 278(44), 43744-43754.

  • Muramatsu, T., Muramatsu, H., Kaneda, N., and Sugahara, K. (2003)
    Recognition of glycosaminoglycans by midkine.
    Methods in Enzymol. (Lee, Y.C., and . Lee R. T. eds.), Academic Press, New York, 363, 365-376.

  • Kim, B-T., Kitagawa, H., Tanaka, J., Tamura, J., and Sugahara, K. (2003)
    In vitro heparan sulfate polymerization: crucial roles of core protein moieties of primer substrates in addition to the EXT1-EXT2 interaction.
    J. Biol. Chem., 278(43), 41618-41623.

  • Sugahara, K., Mikami, T., Uyama, T., Mizuguchi, S., Nomura, K., and Kitagawa, H. (2003)
    Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate (comprehensive review).
    Curr. Opin. Struct. Biol., 13, 612-620.

  • Mikami, T., Mizumoto, S., Kago, N., Kitagawa, H., and Sugahara K. (2003)
    Specificities of three distinct human chondroitin/dermatan N- acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor: implication of differential roles in dermatan sulfate biosynthesis.
    J. Biol. Chem., 278(38), 36115-36127.

  • Maeda, N., He, J., Yajima, Y., Mikami, T., Sugahara, K., and Yabe, T. (2003)
    Heterogeneity of the chondroitin sulfate portion of phosphacan/6B4 proteoglycan regulates its binding affinity for pleiotrophin/HB-GAM.
    J. Biol. Chem., 278(37), 35805-35811.

  • Mizuguchi, S., Uyama, T., Kitagawa, H., Nomura, K. H., Dejima, K., Gengyo-Ando, K., Mitani, S., Kohara, Y., Sugahara , K., and Nomura, K. (2003)
    Chondroitin proteoglycans are involved in cell division of Caenorhabditis elegans.
    Nature, 423, 443-448.

  • Kitagawa, H., Izumikawa, T., Uyama, T., and Sugahara, K. (2003)
    Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization.
    J. Biol. Chem., 278(26), 23666-23671.

  • Pedersen, L.C., Dong, J., Taniguchi, F., Kitagawa, H., Krahn, J., Pedersen, L.G., Sugahara, K., and Negishi, M. (2003)
    Crystal structure of an a1, 4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis.
    J. Biol. Chem., 278(16), 14420-14428.

  • Kim, B.-T., Tsuchida, K., Lincecum, J., Kitagawa, H., Bernfield, M., and Sugahara, K. (2003)
    Identification and characterization of three Drosophila melanogaster glucuronyltransferases responsible for the synthesis of the conserved glycosaminoglycan-protein linkage region of proteoglycans. Two novel homologs exhibit broad specificity toward oligosaccharides from proteoglycans, glycoproteins, and glycosphingolipids.
    J. Biol. Chem., 278 (11), 9116-9124.

  • Uyama, T., Kitagawa, H., Tanaka, J., Tamura, J., Ogawa, T., and Sugahara K. (2003)
    Molecular cloning and expression of a second chondroitin N- acetylgalactosaminyltransferase involved in the biosynthetic initiation and elongation of chondroitin/dermatan sulfate.
    J. Biol. Chem., 278 (5), 3072-3078.

  • Yamada, S. and Sugahara, K. (2003)
    Preparation of oligosaccharides from sulfated glycosaminoglycans using bacterial enzymes.
    In Capillary Electrophoresis of Carbohydrates (Thibault, P., and Honda, S., eds.). Meth. Mol. Biol. 213, pp. 71-78. Humana Press Inc., Totowa, New Jersey
目 TOP
2002
  • Sugahara, K., and Kitagawa, H. (2002)
    Heparin and heparan sulfate biosynthesis (comprehensive review).
    IUBMB Life, 54, 163-175

  • Deepa, S. S., Umehara, Y., Higashiyama, S., Itoh N., and Sugahara , K. (2002)
    Specific interactions of oversulfated chondroitin sulfate E with various heparin- binding growth factors. Implications as a physiological binding partner.
    J. Biol. Chem., 277 (46), 43707-43716

  • Okada, Y., Yamada, S., Toyoshima, M., Dong, J., Nakajima, M., and Sugahara, K. (2002)
    Structural Recognition by Recombinant Human Heparanase That Plays Critical Roles in Tumor Metastasis: HIERARCHICAL SULFATE GROUPS WITH DIFFERENTIAL EFFECTS AND THE ESSENTIAL TARGET DISULFATED TRISACCHARIDE SEQUENCE
    J. Biol. Chem., 277 (45), 42488-42495

  • Kim, B.-T., Kitagawa, H., and Sugahara, K. (2002)
    Hereditary multiple exostoses: association with EXT family genes and defective heparan sulfate biosynthesis (comprehensive review).
    Connective Tissue, 34 (3) 261-265

  • Sugahara, K., and Yamada, S. (2002)
    Analysis of chondroitin sulfate. The repeating disaccharide region for specific protein binding and the protein attachment region critical in biosynthesis.
    In "Analytical techniques to evaluate the structure and functions of natural polysaccharides, glycosaminoglycans" (Volpi, N., ed.). pp. 79-114, Research Signpost, Trivandrum, Kerala, India.

  • Yamada, S., Okada, Y., Ueno, M., Iwata, S., Deepa, S.S., Nishimura, S., Fujita, M., Van Die, I., Hirabayashi, Y., and Sugahara, K. (2002)
    Determination of the glycosaminoglycan-protein linkage region oligosaccharide structures of proteoglycans from Drosophila melanogaster and Caenorhabditis elegans.
    J. Biol. Chem., 277 (35), 31877-31886

  • Kim, B.-T., Kitagawa, H., Tamura, J., Kusche-Gullberg, M., Lindahl, U., and Sugahara, K. (2002)
    Demonstration of a novel gene DEXT3 of Drosophila melanogaster as the essential N-acetylglucosamine transferase in the heparan sulfate biosynthesis: chain initiation and elongation.
    J. Biol. Chem., 277 (16), 13659-13665

  • Kawashima, H., Atarashi, K., Hirose, M., Hirose, J., Yamada, S., Sugahara, K., and Miyasaka, M. (2002)
    Oversulfated chondroitin/dermatan sulfates containing GlcAb1/IdoAa1- 3GalNAc(4,6-O-disulfate) interact with L- and P-selectin and chemokines.
    J. Biol. Chem., 277 (15), 12921-12930

  • Uyama, T., Kitagawa, H., Tamura, J., and Sugahara K. (2002)
    Molecular Cloning and Expression of Human Chondroitin N- Acetylgalactosaminyltransferase: The Key Enzyme for Chain Initiation and Elongation of Chondroitin/Dermatan Sulfate on the Protein Linkage Region Tetrasaccharide Shared by Heparin/Heparan Sulfate.
    J. Biol. Chem., 277 (11), 8841-8846
目 TOP
2001

  • Kitagawa, H., Uyama, T., and Sugahara, K. (2001)
    Molecular cloning and expression of a human chondroitin synthase.
    J. Biol. Chem., 276 (42), 38721-38726

  • Kinoshita, A., Yamada, S., Haslam, S.M., Morris, H.R., Dell, A., and Sugahara, K. (2001)
    Isolation and structural determination of novel sulfated hexasaccharides from squid cartilage chondroitin sulfate E that exhibits neuroregulatory activities.
    Biochemistry, 40 (42), 12654-12665

  • Tsuchida, K., Shioi, J., Yamada, S., Boghosian, G., Wu, A., Gai, H., Sugahara, K., and Robakis N.K. (2001)
    Appican, the proteoglycan form of the amyloid precursor protein, contains the chondroitin sulfate E motif in the repeating disaccharide region and a 4-O-sulfated galactose residue in the linkage region.
    J. Biol. Chem., 276 (40), 37155-37160

  • Kitagawa, H., Taoka, M., Tone, Y., Sugahara, K. (2001)
    Human glycosaminoglycan glucuronyltransferase I gene and a related processed pseudogene. Genomic structure, chromosomal mapping, and characterization.
    Biochem. J., 358 (3), 539-546

  • Kim, B.-T., Kitagawa, H., Tamura, J., Saito, T., Kusche- Gullberg, M., Lindahl, U., and Sugahara, K. (2001)
    The human tumor suppressor EXT gene family members, EXTL1 and EXTL3, encode alpha 1,4-N- acetylglucosaminyltransferases involved in heparan sulfate/heparin biosynthesis.
    Proc. Natl. Acad. Sci. USA, 98 (13), 7176-7181

  • Ueno, M., Yamada, S., Zako, M., Bernfield, M., and Sugahara, K. (2001)
    Structural characterization of heparan sulfate and chondroitin sulfate of syndecan- 1 purified from normal murine mammary gland epithelial cells. Common phosphorylation of xylose and differential sulfation of galactose in the protein linkage region tetrasaccharide sequence.
    J. Biol. Chem., 276 (31), 29134-29140

  • Tamura, J., Urashima, H., Tsuchida, K., Kitagawa, H., and Sugahara, K. (2001)
    Synthesis of linear-type chondroitin clusters having a C8 spacer between disaccharide moieties and enzymatic transfer of D-glucuronic acid to the artificial glycans.
    Carbohydr. Res., 322 (1), 41-51

  • Kitagawa, H., Egusa, N., Tamura, J., Kusche-Gullberg, M., Lindahl, U., and Sugahara, K. (2001)
    rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha 1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate.
    J. Biol. Chem., 276 (7), 4834-4838

  • Sakaguchi, H., Watanabe, M., Ueoka, C., Sugiyama, E., Taketomi, T., Yamada, S., and Sugahara, K. (2001)
    Isolation of oligosaccharides from the chondroitin/dermatan sulfate-protein linkage region as reducing sugar chains and preparation of the analytical probes by fluorescent labeling with 2- aminobenzamide.
    J. Biochem., 129 (19), 107-118
    • 目 TOP
    2000

  • Ueoka, C., Kaneda, N., Okazaki, I., Nadanaka, S., Muramatsu, T., and Sugahara, K. (2000)
    Inhibition of the midkine-mediated neural cell adhesion to culture substrates by chondroitin sulfate E. Specific molecular interactions of midkine with chondroitin sulfate E.
    J. Biol. Chem., 275 (48), 37407-37413

  • Sugahara, K. and Yamada, S. (2000)
    Structure and function of oversulfated chondroitin sulfate variants: unique sulfation patterns and neuroregulatory activities (comprehensive review).
    Trends in Glycoscience and Glycotechnology, 12 (67), 321- 349


  • Pedersen, L.C., Tsuchida, K., Kitagawa, H., Sugahara, K., and Negishi M. (2000)
    Heparan/chondroitin sulfate biosynthesis: structure and mechanism of human glucuronyltransferase I.
    J. Biol. Chem., 275 (44), 34580-34585


  • Sakaguchi, H., Kitagawa, H. and Sugahara, K. (2000)
    Functional expression and genomic structure of human N- acetylglucosamine-6-O- sulfotransferase that transfers sulfate to b-N-acetylglucosamine at the nonreducing end of an N- acetyllactosamine sequence.
    Biochim Biophys Acta, 1523 (2-3), 269-276


  • Sugahara, K., and Kitagawa, H. (2000)
    Recent advances in the study of the biosynthesis and functions of sulfated glycosaminoglycans.
    Current opinion in structural biology, 10 (5), 518-527


  • Senay, C., Lind, T., Muguruma, K., Tone, Y., Kitagawa, H., Sugahara, K., Lidholt, K., Lindahl, U., and Kusche-Gullberg, M. (2000)
    Association of the EXT1 and EXT2 proteins in heparan sulfate biosynthesis.
    EMBO reports, 1 (3), 282-286


  • Kishibe, J., Yamada, S., Okada, Y., Sato, J., Ito, A., Miyazaki, K., and Sugahara, K. (2000)
    Structural requirements of heparan sulfate for the binding to the tumor-derived adhesion factor/angiomodulin that induces cord-like structures to ECV-304 human carcinoma cells.
    J. Biol. Chem., 275(20), 15321-15329


  • Kitagawa, H., Fujita, M., Ito, N., and Sugahara, K. (2000)
    Molecular cloning and expression of a novel chondroitin 6-O- sulfotransferase.
    J. Biol. Chem., 275(28), 21075-21080
    		•
    目 TOP
    1999
    • Sato, J., Hasegawa, S., Akaogi, K., Yasumitsu, H., Yamada, S., Sugahara, K., and Miyazaki, K. (1999)
      Identification of cell binding site of angiomodulin (AGM/TAF/Mac25), that interacts with heparan sulfate on cell surface.
      J. Cell. Biochem., 75(2), 187-195


    • Yamada, S., Van Die, I., Van den Eijnden, D.H., Yokota, A., Kitagawa, H., and Sugahara, K. (1999)
      Demonstration of glycosaminoglycans in Caenorhabditis elegans .
      FEBS Letters, 459 (3), 327-331


    • Tone, Y., Kitagawa, H., Oka, S., Kawasaki, T. and Sugahara, K. (1999)
      Characterization of recombinant glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans.
      FEBS Letters, 459 (3), 415-420


    • Clement, A., Sugahara, K., and Faissner, A. (1999)
      Chondroitin sulfatae E promoyes neurite outgrowth of embryonic day 18 hippocampal neurons.
      Neuroscience Letters, 269 (3), 125-128


    • Tsuchida, K., Lind, T., Kitagawa, H., Lindahl, U., Sugahara, K., and Lidholt, K. (1999)
      Purification and characterization of fetal bovine serum N-acetyl-D- galactosaminyltransferase and glucuronyltransferase involved in chondroitin sulfate biosynthesis.
      Eur. J. Biochem., 264 (2),461-467


    • Ueoka, C., Nadanaka, S., Seno, N., Khoo, K.-H., and Sugahara, K. (1999)
      Structural Determination of Novel Tetra- and Hexasaccharide Sequences Isolated from Chondroitin Sulfate H (Oversulfated Dermatan sulfate) of Hagfish Notochord.
      Glycoconjugate J., 16 (6), 291-305


    • Nadanaka, S., Fujita, M., and Sugahara, K. (1999)
      Demonstration of a Novel Sulfotransferase in Fetal Bovine Serum, Which Transfers Sulfate to the C6 Position of the GalNAc Residue in the Sequence Iduronic acida1- 3GalNAcb1- 4Iduronic acid in Dermatan Sulfate.
      FEBS Letters, 452 (3), 185-189


    • Nadanaka, S., Kitagawa, H., Goto, F., Tamura, J., Neumann, K.W., Ogawa, T., and Sugahara, K. (1999)
      Involvement of the Core Protein in the First b-N-Acetylgalactosamine Transfer to the Glycosaminoglycan-Protein Linkage Region Tetrasaccharide and in the Subsequent Polymerization: The critical determining step for chondroitin sulfate biosynthesis.
      Biochem. J., 340 (2), 353-357


    • Kitagawa, H.,Shimakawa, H., and Sugahara, K.(1999)
      The Tumor Suppressor EXT-like Gene EXTL2 Encodes an a1,4-N- Acetylhexosaminyltransferase That Transfers N-Acetylgalactosamine and N-Acetylglucosamine to the Common Glycosaminoglycan- Protein Linkage Region. The key enzyme for the chain initiation of heparan sulfate
      J. Biol Chem., 274 (20), 13933-13937


    • Kinoshita, A., and Sugahara, K. (1999)
      Microanalysis of Glycosaminoglycan-Derived Oligosaccharides Labeled with the Fluorophore 2- Aminobenzamide by High-Performance Liquid Chromatography: Application to Disaccharide Composition Analysis and Exo-sequencing of Oligosaccharides.
      Anal. Biochem., 269 (2), 367-378


    • Tsuda, H., Yamada, S., Miyazono, H., Morikawa, K., Yoshida, K., Goto, F., Tamura, J., Neumann, K. W., Ogawa, T., and Sugahara, K. (1999)
      Substrate specificity studies of Flavobacterium chondroitinase C and heparitinases towards the glycosaminoglycan-protein linkage region: Use of a sensitive analytical method developed by chromophore-labeling of linkage glycoserines using dimethylaminoazobenzenesulfonyl chloride.
      Eur. J. Biochem., 262 (1), 127-133


    • Kitagawa, H., Kano, Y., Shimakawa, H., Goto, F., Ogawa, T., Okabe, H. and Sugahara, K.(1999)
      Identification and characterization of a novel UDP-GalNAc: GlcAb- Ra1,4-N acetylgalactosaminyltransferase from a human sarcoma cell line.
      Glycobiology, 9 (7), 697-703


    • Yamada, S., Sakamoto, K., Tsuda, H., Yoshida, K., Sugiura, M. and Sugahara, K. (1999)
      Structural studies of octasaccharides derived from the low-sulfated repeating disaccharide region and octasaccharide serines derived from the protein linkage region of porcine intestinal heparin.
      Biochemistry, 38 (2), 838-847
    目 TOP
    1998
    • Kae Tsutsumi, K., Shimakawa, H., Kitagawa, H., and Sugahara, K. (1998)
      Functional expression and genomic structure of human chondroitin 6- sulfotransferase.
      FEBS Letters, 441(2), 235-241


    • Yamada, S., Yamane, Y., Sakamoto, K., Tsuda, H. and Sugahara, K. (1998)
      Structural determination of sulfated tetrasaccharides and hexasaccharides containing a rare disaccharide sequence, -3GalNAc(4,6-disulfated)b1-4IdoAa1-, isolated from porcine intestinal dermatan sulfate.
      Eur. J. Biochem., 258 (2), 775-783


    • Nadanaka, S., Kitagawa, K. and Sugahara, K. (1998)
      Demonstration of the immature glycosaminoglycan tetrasaccharide sequence GlcAb1-3Galb1- 3Galb1-4Xyl on recombinant soluble human a-thrombomodulin. A possible mechanism generating "part- time" proteoglycans.
      J. Biol Chem., 273 (50), 33728-33734


    • Clement, A. M., Nadanaka, S., Masayama, K., Mandl, C., Sugahara, K. and Faissner, A. (1998)
      The DSD-1-epitope depends on sulfation, correlates with chondroitin sulfate D motifs, and is sufficient to promote neurite outgrowth.
      J. Biol. Chem., 273 (43), 28444-28453


    • Yamada, S., Watanabe, M., and Sugahara, K. (1998)
      Conversion of N-sulfated glucosamine to N-sulfated mannosamine in an unsaturated heparin disaccharide by non-enzymatic, base-catalyzed C-2 epimerization during enzymatic oligosaccharide preparation.
      Carbohyd. Res., 309 (3), 261-268.


    • Yamada, S. and Sugahara, K. (1998)
      Structure of oligosaccharides isolated from heparan sulfate/heparin and substrate specificities of the degrading enzymes of bacterial origin (comprehensive review).
      Trends in Glycoscience and Glycotechnology, 10 (52), 95- 123.


    • Yamane, Y., Tohno-oka, R., Yamada, S., Furuya, S., Shiokawa, K., Hirabayashi, Y., Sugino, H. and Sugahara, K. (1998)
      Molecular characterization of Xenopus embryo heparan sulfate: differential structural requirements for the specific binding to basic fibroblast growth factor and follistatin.
      J. Biol. Chem., 273 (13), 7375-7381.


    • Kitagawa, H., Tone, Y., Tamura, J., Neumann, K.W., Ogawa, T., Oka, S., Kawasaki, T., and Sugahara, K. (1998)
      Molecular cloning and expression of glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycasns.
      J. Biol. Chem., 273 (12), 6615-6618.


    • Nadanaka, S., Clement, A., Masayama, K., Faissner, A. and Sugahara, K. (1998)
      Characteristic hexasaccharide sequences in octasaccharides derived from shark cartilage chondroitin sulfate D with neurite outgrowth promoting activity.
      J. Biol. Chem., 273 (6), 3296-3307.


    • Watanabe, M., Tsuda, H., Yamada, S., Shibata, Y., Nakamura, T. and Sugahara, K. (1998)
      Characterization of heparinase from an oral bacterium Prevotella heparinolytica.
      J. Biochem., 123 (2), 283-288.


    • Yamada, S., Yamane, Y., Tsuda, H., Yoshida, K. and Sugahara, K. (1998)
      A major common trisulfated hexasaccharide core sequence, hexuronic acid(2- sulfate)- glucosamine(N-sulfate)-iduronic acid-N-acetylglucosamine-glucuronic acid- glucosamine(N-sulfate), isolated from the low sulfated irregular region of porcine intestinal heparin.
      J. Biol. Chem., 273 (4), 1863-1871.
    目次に TOP
    1997
    • Kitagawa, H., Tsutsumi, K., Tone, Y. and Sugahara, K. (1997)
      Developmental regulation of the sulfation profile of chondroitin sulfate chains in the chicken embryo brain.
      J. Biol. Chem., 272 (50), 31377-31381.


    • Kitagawa, H., Oyama, M., Masayama, K., Yamaguchi, Y. and Sugahara, K. (1997)
      Structural variations in the glycosaminoglycan-protein linkage region of recombinant decorin expressed in chinese hamster ovary cells.
      Glycobiology, 7 (8), 1175-1180.


    • Kitagawa, H., Ujikawa, M., Tsutsumi, K., Tamura, J., Neumann, K., Ogawa, T. and Sugahara, K. (1997)
      Characterization of serum b-glucuronyltransferase involved in chondroitin sulfate biosynthesis.
      Glycobiology, 7 (7), 905-911.


    • Kinoshita, A., Yamada, S., Haslam, S. M., Morris, H. R., and Dell, A. Sugahara, K. (1997)
      Novel tetrasaccharides isolated from squid cartilage chondroitin sulfate E contain unusual sulfataed disaccharide units GlcA(3-O-sulfate)b1-3GalNAc(6-O-sulfate) or GlcA(3- O-sulfate)b1- 3GalNAc(4,6-O- disulfate).
      J. Biol. Chem., 272 (32), 19656-19665.


    • Kitagawa, H., Tanaka, Y., Yamada, S., Seno, N., Haslam, S. M., Morris, H. R., Dell, A. and Sugahara, K. (1997)
      A novel pentasaccharide sequence GlcA(3-sulfate)(b1-3)GalNAc(4- sulfate)(b1- 4)(Fuca1- 3)GlcA(b1-3)GalNAc(4-sulfate) in the oligosaccha-rides isolated from king crab cartilage chondroitin sulfate K and its differential susceptibility to chondroitinases and hyaluronidase.
      Biochemistry, 36 (13), 3998-4008.


    • Kitagawa, H., Tsutsumi, K., Ujikawa, M., Goto, F., Tamura, J., Neumann, K., Ogawa, T. and Sugahara, K.  (1997)
      Regulation of chondroitin sulfate biosynthesis by specific sulfation: acceptor specificity of serum b- GalNAc transferase revealed by structurally-defined oligosaccharides.
      Glycobiology, 7 (4), 531-537.


    • Lidholt, S., Fjelstad, M., Lindahl, U., Goto, F., Ogawa, T., Kitagawa, H. and Sugahara, K. (1997)
      Assessment of glycosaminoglycan-protein linkage tetrasaccharides as acceptors for GalNAc- and GlcNAc-transferases from mouse mastocytoma.
      Glycoconjugate J., 14 (6), 737-742.


    • Nadanaka, S. and Sugahara, K. (1997)
      The unusual tetrasaccharide sequence GlcAb1-3GalNAc(4- sulfate)b1- 4GlcA(2- sulfate)b1- 3GalNAc(6-sulfate) found in the hexasaccharides prepared by testicular hyaluronidase digestion of shark cartilage chondroitin sulfate D.
      Glycobiology, 7 (2), 253-263.
    目次に TOP
    1996
    • Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R. and Dell, A. (1996)
      Novel sulfated oligosaccharides containing 3-O-sulfated glucuronic acid from king crab cartilage chondroitin sulfate K: the unexpected degradation by chondroitinase ABC.
      J. Biol. Chem., 271 (43), 26745-26754.


    • De Beer, T., Inui, A., Tsuda, H., Sugahara, K., and Vliegenthart, J. F. G. (1996)
      Polydispersity in sulfation profile of oligosaccharide alditols isolated from the protein-linkage region and the repeating disaccharide region of chondroitin 4-sulfate of bovine nasal septal cartilage.
      Eur. J. Biochem., 240 (3), 789-797.


    • Sugahara, K. and Kojima, T. (1996)
      Specificity studies of the bacterial sulfatases using the structurally defined sulfated oligosaccharides isolated from shark cartilage chondroitin sulfate D.
      Eur. J. Biochem., 239 (3), 865-870.


    • Sugahara, K., Nadanaka, S., Takeda, K. and Kojima, T. (1996)
      Structural analysis of unsaturated hexasaccharides isolated from shark cartilage chondroitin sulfate D: substrates for exolytic action of chondroitinase ABC.
      Eur. J. Biochem., 239 (3), 871-880.


    • Akaogi, K., Okabe, Y., Sato, J., Nagashima, Y., Yasumitsu, H., Sugahara, K. and Miyazaki, K. (1996)
      Specific accumulation of tumor-derived adhesion factor in tumor blood vessels and in capillary tube-like structures of cultured vascular endothelial cells.
      Proc. Natl. Acad. Sci. USA, 93 (16), 8384-8389.


    • Tsuda, H., Yamada, S., Yamane, Y., Yoshida, K., Hopwood, J. J. and Sugahara, K.(1996)
      Structures of five sulfated hexasaccharides prepared from porcine intestinal heparin using bacterial heparitinase: structural variants with apparent biosynthetic precursor-product relationships for the antithrombin III-binding site.
      J. Biol. Chem., 271(18), 10495-10502.


    • Kitagawa, H., Ujikawa, M. and Sugahara, K. (1996)
      Developmental changes in serum UDP-GlcA: chondroitin glucuronyltransferase activity.
      J. Biol. Chem., 271(12), 6583-6585.


    • Sugahara, K., Tanaka, Y. and Yamada, S. (1996)
      Preparation of a series of sulfated tetrasaccharides from shark cartilage chondroitin sulfate D using testicular hyaluronidase and structure determination by 500-MHz 1H NMR spectroscopy.
      Glycoconjugate J., 13 (4), 609-619.
    目次に TOP
    1995
    • Kitagawa, H., Kinoshita, A. and Sugahara, K. (1995)
      Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2- aminoacridone by capillary electrophoresis and high-performance liquid chromatography.
      Anal. Biochem., 232 (1), 114-121.


    • Yamada, S., Oyama, M., Yuki, Y., Kato, K. and Sugahara, K. (1995)
      The uniform galactose 4-sulfate structure in the carbohydrate protein linkage region of human urinary trypsin inhibitor.
      Eur. J. Biochem., 233 (2), 687-693.


    • Sugahara, K., Tsuda, H., Yoshida, K., Yamada, S., de Beer, T. and Vliegenthart, J. F. G. (1995)
      Structure determination of the octa-and decasaccharide sequences isolated from the carbohydrate- protein linkage region of porcine intestinal heparin.
      J. Biol. Chem., 270 (39), 22914-22923.


    • Kitagawa, H., Tanaka, Y., Tsuchida, K., Goto, F., Ogawa, T., Lidholt, K., Lindahl., U. and Sugahara, K.(1995)
      GalNAc transfer to the common carbohydrate-protein linkage region of sulfated glycosaminoglycans: identification of UDP-GalNAc: chondro- oligosaccharide a-N- acetylgalactosaminyltransferase in bovine serum.
      J. Biol. Chem., 270 (38), 22190-22195.


    • Furuya, S., Sera, M., Tohno-oka, R., Sugahara, K., Shiokawa, K. and Hirabayashi, Y. (1995)
      Elimination of heparan sulfate by heparitinases induces abnormal mesodermal and neural formation in Xenopus embryos.
      Develop. Growth Differ., 37 (3), 337-346.


    • Yamada, S., Oyama, M., Kinugasa, H., Nakagawa, T., Kawasaki, T., Nagasawa, S., Khoo, K.-H., Morris, H. R., Dell, A., and Sugahara, K. (1995)
      The sulfated carbohydrate-protein linkage region isolated from chondroitin 4- sulfate chains of inter-a-trypsin inhibitor in human plasma.
      Glycobiology, 5 (3), 335-341.


    • Kitagawa, H., Tsuchida, K., Ujikawa, M. and Sugahara, K. (1995)
      Detection and characterization of UDP-GalNAc: chondroitin N acetylgalactosaminyltransferase in bovine serum using a simple assay method.
      J. Biochem., 117 (5), 1083-1087.


    • Yamada, S., Murakami, T., Tsuda, H., Yoshida, K. and Sugahara, K. (1995)
      Isolation of the porcine heparin tetrasaccharides with glucuronate 2-O-sulfate. Heparinase cleaves glucuronate 2-O-sulfate containing disaccharides in highly sulfated blocks in heparin.
      J. Biol. Chem., 270 (15), 8696-8705.


    • Sugahara, K., Ohkita, Y., Shibata, Y., Yoshida, K. and Ikegami, A. (1995)
      Structural studies on the hexasaccharide alditols isolated from the carbohydrate- protein linkage region of dermatan sulphate proteoglycans of bovine aorta. Demonstration of iduronic acid-containing components.
      J. Biol. Chem., 270 (13), 7204-7212.

    目次に TOP